LOCUS       sequence-157277-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting murine CD45.2 region 3. Co-expresses 
            SpCas9-2A-EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Kornete M, Marone R, Jeker LT
  TITLE     Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary
            T Cells.
  JOURNAL   J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 
            10.4049/jimmunol.1701121.
  PUBMED    29445007
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121."
COMMENT     Sequence Label: p206_LTJ_sgRNACD45.2_R3
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        33..108
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        205..490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ggacgaaaca ccgtaggtcc ggacaaggtc aagttttaga gctagaaata gcaagttaaa
       61 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
      121 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg
      181 cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc cgcctggctg
      241 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc
      421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      481 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      541 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      601 gggggggggg ggscgcgcgc caggcggggc ggggcggggc kaggggcggg gcgggkcgag
      661 gcgkawaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
      721 gaggc
//
