LOCUS       sequence-157285-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a Myc tagged version of the BlaM-Vpr fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pMM310-Myc
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..123
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             239..617
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(273..292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct tggtaccacc atggaacaga aactgattag cgaagaagat
      121 ctggacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      181 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      241 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      301 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      361 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      421 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      481 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      541 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      601 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      661 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      721 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      781 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagt
//
