LOCUS       sequence-157289-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving Avi-tagged protein containing Cerulean 
            protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 
            2012); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-Avi-Cerulean-Rpl10a
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4..22
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             651..659
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(785..806)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggttaatacg actcactata gggagtccgg atacgtatga attctactag tagaggcgct
       61 gtggttttcc catggtgctc ttgatgtaaa gagctctgac gttttgccag ttcttcttca
      121 gcagagacac caggaagttg acagccaggt ggatgttgta gaccagctct tcctcactca
      181 tcttcacatg acccacggcc acagccagac acagaacctt cttcatctgg aatttgatgg
      241 tggatttcac ctcatccacc ttggtgccca ggttctcatt atgggtgagc agagagggaa
      301 actttcctgc cttgttgagg ccagggccca ggatacgagg aatctgcttg atcagagact
      361 cagaggccag gaaagcatcg tacttctttg ccagcttctt gaccagtttc ttgttcttat
      421 tgagcttctt aagagcctca atgtccatgt gtggcagttc agcggccttt gcttcatcac
      481 aatgctgctg gtctccaaga acgcagacag agaacttggg ccgcggggtg gtcttcagcc
      541 tgacagtgcc tgagaaacgc ttgtccttct gagggtcgta gttcttcaag ctgatctgga
      601 gttccacggt ttcaagaaac tttctcttct tgcgacggga accggcctgg acctccttaa
      661 cggcctcgta caacgtgtct ctcgagacct tgctgaattc gaagcttgag ctcgagatct
      721 gagtccggcc cgatgcagcg gccttgtaca gctcgtccat gccgagagtg atcccggcgg
      781 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct
      841 tggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      901 tgttctgctg gtagtggtcg
//
