LOCUS       sequence-157293-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HPV 16E6no* in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pNCMV 16E6no*
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..184
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             197..223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(784..803)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    849..904
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(903..922)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
       61 ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct
      121 gtctcatcat tttggcaaag aattcctcga cggatctatg gactacaagg atgacgatga
      181 caagctcgat ggaggatacc catacgatgt tccagattac gctgatatcg gatcctttca
      241 ggacccacag gagcgaccca gaaagttacc acagttatgc acagagctgc aaacaactat
      301 acatgatata atattagaat gtgtgtactg caagcaacag ttactgcgac gcgagctata
      361 tgactttgct tttcgggatt tatgcatagt atatagagat gggaatccat atgctgtatg
      421 tgataaatgt ttaaagtttt attctaaaat tagtgagtat agacattatt gttatagttt
      481 gtatggaaca acattagaac agcaatacaa caaaccgttg tgtgatttgt taattaggtg
      541 tattaactgt caaaagccac tgtgtcctga agaaaagcaa agacatctgg acaaaaagca
      601 aagattccat aatataaggg gtcggtggac cggtcgatgt atgtcttgtt gcagatcatc
      661 aagaacacgt agagaaaccc agctgtaaag atccgtcgag gaattcactc ctcaggtgca
      721 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
      781 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
      841 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
      901 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcaga
//
