LOCUS       sequence-157298-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving N-term Avi-tagged protein containing 
            Cerulean protein fused to the carboxy-terminal domain of avian Ran 
            GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-Avi-Cerulean-RanGAP
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(33..44,45..182)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    274..408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(311..330)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     365..384
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     415..434
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        415..433
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agggcgattg ggtaccggca tatggttctt gacagaggtg taaaaagtac tcaaaaattt
       61 tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag taaaagtatc
      121 tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg tacttgagta
      181 ttagatctgc atgcgtttaa acccgggata tcaaaaaacc tcccacacct ccccctgaac
      241 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      301 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      361 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcac cggttaatac
      421 gactcactat agggagtccg gatacgtatg aattcattat tacagctggt ggagggtttg
      481 taaaagcatg tggcgggcaa aggaacagga gtcgagagca cggtttggtt ttgtgacaaa
      541 agcagagaga actggggcca gtgatttagg gaaataatcc tgctggacca tatgattcaa
      601 agtcataaga ataccataga ggctcgggac agctttgatc ttctcttcac tcttaagtag
      661 ccccatgtgg atcaagagtc ttgtgatgaa tgcatctgaa ttaaatgtgg cggaagagaa
      721 ggcttttttc atcaaggcat ctgctgtttc atgcactgct gtttttactt gggcttcatc
      781 tttgaagact gaagatatcc ttaagagagc tgtaactact ttctctacat cagatgtatc
      841 tgtctgctga gctatcagca cagaacactt tggccctagc cgcagcagct tctctggtga
      901 tgggaaagca aggaacgtgg caacgtccac aggaggagat ggcactggag ttgaatcctg
      961 tgaaccaatt atcttcttag gagccataat cgatgcagcg gccttgtaca
//
