LOCUS       sequence-157299-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tissue-specific multiplex gRNA plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Port F, Bullock SL
  TITLE     Augmenting CRISPR applications in Drosophila with tRNA-flanked 
            sgRNAs.
  JOURNAL   Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 
            2016 Sep 5.
  PUBMED    27595403
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3972"; journalName: "Nat Methods"; date: "2016-10"; 
            volume: "13"; issue: "10"; pages: "852-4"
COMMENT     Sequence Label: pCFD6
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    153..186
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    199..629
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    675..769
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 aagtgacatc cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg tgggcataat
       61 agtgttgttt atatatatca aaaataacaa ctataataat aagaatacat ttaatttaga
      121 aaatgcttgg atttcactgg aactaggcta gcataacttc gtataatgta tgctatacga
      181 agttatgcta gcggatcctg gccacgtaat aagtgtgcgt tgaatttatt cgcaaaaaca
      241 ttgcatattt tcggcaaagt aaaattttgt tgcatacctt atcaaaaaat aagtgctgca
      301 tactttttag agaaaccaaa taatttttta ttgcataccc gtttttaata aaatacattg
      361 cataccctct tttaataaaa aatattgcat actttgacga aacaaatttt cgttgcatac
      421 ccaataaaag attattatat tgcatacccg tttttaataa aatacattgc ataccctctt
      481 ttaataaaaa atattgcata cgttgacgaa acaaattttc gttgcatacc caataaaaga
      541 ttattatatt gcataccttt tcttgccata ccatttagcc gatcaattgt gctcggcaac
      601 agtatatttg tggtgtgcca accaacaaca gatccaagct ggccgcggct cgatccgctt
      661 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      721 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggaagcttgc
      781 atgcctgcag gtcggagtac tgtcctccga gcggagtact g
//
