LOCUS       sequence-157301-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving Avi-tagged protein containing Cerulean 
            protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 
            2012); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-Avi-Cerulean-Rpl10a
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(32..43,44..181)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    273..407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(310..329)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     364..383
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     414..433
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        414..432
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgattgg gtaccggcat atggttcttg acagaggtgt aaaaagtact caaaaatttt
       61 actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct
      121 ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat
      181 tagatctgca tgcgtttaaa cccgggatat caaaaaacct cccacacctc cccctgaacc
      241 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      301 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
      361 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcacc ggttaatacg
      421 actcactata gggagtccgg atacgtatga attctactag tagaggcgct gtggttttcc
      481 catggtgctc ttgatgtaaa gagctctgac gttttgccag ttcttcttca gcagagacac
      541 caggaagttg acagccaggt ggatgttgta gaccagctct tcctcactca tcttcacatg
      601 acccacggcc acagccagac acagaacctt cttcatctgg aatttgatgg tggatttcac
      661 ctcatccacc ttggtgccca ggttctcatt atgggtgagc agagagggaa actttcctgc
      721 cttgttgagg ccagggccca ggatacgagg aatctgcttg atcagagact cagaggccag
      781 gaaagcatcg tacttctttg ccagcttctt gaccagtttc ttgttcttat tgagcttctt
      841 aagagcctca atgtccatgt gtggcagttc agcggccttt gcttcatcac aatgctgctg
      901 gtctccaaga acgcagacag agaacttggg ccgcggggtg gtcttca
//
