LOCUS       sequence-157305-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52/Bag5_1_447
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..91
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             92..109
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatatagg
       61 atctatgcac caccatcacc accatcacca cttggtgccc cgcggatcca tggacatggg
      121 taaccaacat cctagcatca gtcgccttca agaaattcag aaagaagtga agtcagttga
      181 acaacaggtg ataggcttca gtggcctgag cgacgacaag aactataaga agctagaacg
      241 tatcctgact aaacaactct tcgagatcga tagcgttgac acggagggta aaggagacat
      301 ccaacaagct aggaaacgcg ctgctcagga gaccgaacgt ctattaaagg agctagagca
      361 gaacgctaac catcctcatc gtattgagat ccagaacatc tttgaagaag cccaatcatt
      421 agttagggag aagatcgttc ctttctacaa cggaggcaac tgcgttactg acgagttcga
      481 ggagggtatc caagacatca tcctgcgtct gacccacgtt aagaccggcg gtaagatatc
      541 acttaggaag gctaggtacc atacccttac caaaatctgc gctgttcaag aaataatcga
      601 agattgcatg aagaaacaac cctcactccc gctgagtgaa gatgctcatc cctcagtagc
      661 taaaatcaac ttcgtgatgt gcgaggtgaa caaggcccgt ggtgtcttaa ttgctttgtt
      721 aatgggcgtg aacaataacg aaacatgcag gcacctgagc tgcgtgctgt caggtctcat
      781 tgccgattta gacgctttag acgtttgcgg tcgtaccgaa atccgtaact ataggcgtga
      841 agtagttgaa gacattaata agttacttaa gtacttg
//
