LOCUS       sequence-157309-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and mCherry protein; flanked by Tol2 
            sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-cytoBirA-2a-mCherry
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..105)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ctccttgatg atggccatgt tatcctcctc gcccttcgac accatacttc cgggcccggg
       61 attttcctcc acgtccccgc atgttagaag acttcccctg ccctctcctg atccgatatc
      121 tttttctgca ctacgcaggg atatttcacc gcccatccag ggttttatta ttccatcctg
      181 ctcaagtaat aaagccccct gtttgtctat tccgcgtgaa atgccaaata tttctttatc
      241 accaatgata agtttcactg ggcgattaat aaaattatcc agcttttccc agcgcgacag
      301 ataaggtgcc aatccttctt gttcgaagag ttccaacgca gcacgtaatt cacgtattag
      361 catggccgcc aacgtattac gatcgagatt gatccccgct tcctgcagcg tgatccaccc
      421 ctgattaacg acactctctt caacacggcg cattgccatg ttgatcccgg ctccaatgac
      481 tatttgcgcc gcatcgccag ttttgccagt cagctccacc agaatgcctg ccagcttgcg
      541 atcctgcaga tagaggtcat taggccattt aacacgaact ttatctgcac ccagcttgcg
      601 taatacttcc gccatcacga taccgataac cagacttaaa ccaatcgccg ccgccgggcc
      661 ttgttccaga cgccagaaca tcgacaaata taagtttgcg ccaaaaggcg aaaaccattt
      721 ccgaccccgg cgaccacggc cagcctgctg gtattctgca atgcaagcat cgcccgattt
      781 aagctctccg atacgatcaa gaaggtactg attcgtggag tcaatcactg gcagcacggc
      841 tacactaccg ccatccagct gacccaatat ctgtttagca ttaagtaact ggataggctc
      901 aggcaggctg tatccttttc ccggaacggt aaagacatca acgccccagt cacgcagtgt
      961 ctgaatgtgt ttattaatag ccgcccggct cattcccagc gtttcaccca actgctcgcc
     1021 aga
//
