LOCUS       sequence-157310-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tissue-specific multiplex gRNA plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Port F, Bullock SL
  TITLE     Augmenting CRISPR applications in Drosophila with tRNA-flanked 
            sgRNAs.
  JOURNAL   Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 
            2016 Sep 5.
  PUBMED    27595403
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3972"; journalName: "Nat Methods"; date: "2016-10"; 
            volume: "13"; issue: "10"; pages: "852-4"
COMMENT     Sequence Label: pCFD6
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    254..323
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    357..787
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggct agaactagtg
       61 tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta tccgcacccg
      121 ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg
      181 agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca
      241 tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct
      301 ggcacccgca ccgcggcttc gagactgtga cctacatcgt cgacactagt ggatccgttg
      361 ttggttggca caccacaaat atactgttgc cgagcacaat tgatcggcta aatggtatgg
      421 caagaaaagg tatgcaatat aataatcttt tattgggtat gcaacgaaaa tttgtttcgt
      481 caacgtatgc aatatttttt attaaaagag ggtatgcaat gtattttatt aaaaacgggt
      541 atgcaatata ataatctttt attgggtatg caacgaaaat ttgtttcgtc aaagtatgca
      601 atatttttta ttaaaagagg gtatgcaatg tattttatta aaaacgggta tgcaataaaa
      661 aattatttgg tttctctaaa aagtatgcag cacttatttt ttgataaggt atgcaacaaa
      721 attttacttt gccgaaaata tgcaatgttt ttgcgaataa attcaacgca cacttattac
      781 gtggccaaat gtttccgcgc gagtttcccg acgacatgtc gcgcacgttc ctgactgcgt
      841 actttgagag tttcgacaac accacgtcca gagaaatcat agagtttatg cccgagttct
      901 tttcggaaat ggtcgaaacg gacgatgacg ccacgtt
//
