LOCUS       sequence-157311-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with 
            nuclear localization signal (NLS), a Thosea asigna virus peptide 
            (2A), and mCherry protein; flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-NLS-BirA-2a-mCherry
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(8..19,20..207)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 caagaaccag aggtgtaaag tacttgagta attttacttg attactgtac ttaagtatta
       61 tttttgggga tttttacttt acttgagtac aattaaaaat caatactttt acttttactt
      121 aattacattt ttttagaaaa aaaagtactt tttactcctt acaattttat ttacagtcaa
      181 aaagtactta ttttttggag atcacttggg cccggctcga gaccagcaaa gttctagaat
      241 ttgtcgaaac atttatgtta tatatttcct gaaaaaaatt ctgagtaagt tcttaagtgt
      301 tattgccagc aacataaaca acagacggca aaatgaataa atgataacaa agcagtaggc
      361 ttaaataaac ctaattttta taggctgttc tctacaaccc tcaaacagtg attagttttg
      421 tacttataaa cttgcccttt cattcatatt tcaagaaaat tggttcagaa gatctggata
      481 ttctagcagt tgttcaagct catggaggga tcagtgacct gattccacaa tgactaggcc
      541 taatccagaa attagatgac tgtcaacata aaaaggcaca gcactcacta gctgccctat
      601 atattttatt atattttaca tatattattt tatttattta gctctgagtg ctgtactttc
      661 tggttaaaga aaactgctta caacagctaa cctgtactac ctcaggctca gggaatttgg
      721 aacaggtttg tctggtttgt ttctttaacc atgcatgctt gttttcaact atggcaacac
      781 agtcacatgg gacattacag aaatgatttg tcgatgacat gcgacttttc tttaataaag
      841 cgcaaagatc ccaaaaagca aacttttaac aaaaatcata taattatatt ttcaatccag
      901 ctttgtagca actttgtgct gctgttcact ca
//
