LOCUS       sequence-157313-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with 
            nuclear localization signal (NLS), a Thosea asigna virus peptide 
            (2A), and mCherry protein; flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-NLS-BirA-2a-mCherry
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..757)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(325..344)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(764..817)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tctgctcgaa gcggccggcc gccccgactc tagagtcgcg gccgctttac ttgtacagct
       61 cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt
      121 agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct
      181 gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct
      241 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
      301 cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt
      361 tggtgccgcg cagcttcact ttataaatga actcgccatc ttggagggag gagtcctggg
      421 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
      481 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
      541 cgtacatgaa ttgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca
      601 ctttaagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
      661 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
      721 tgatggccat gttatcctcc tcgcccttcg acaccatact tccgggcccg ggattttcct
      781 ccacgtcccc gcatgttaga agacttcccc tgccctctcc tgatccgata tctttttctg
      841 cactacgcag ggatatttca ccgcccatcc agggttttat tattccatcc tgctcaagta
      901 ataaagcccc ctgtttgtct attccgcgtg aaatgccaaa tatttcttta tcaccaatga
      961 taagtttcac tgggcgatta ataaaatta
//
