LOCUS       sequence-157347-        2016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express human caspase 8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Oberst A, Pop C, Tremblay AG, Blais V, Denault JB, Salvesen GS, 
            Green DR
  TITLE     Inducible dimerization and inducible cleavage reveal a requirement 
            for both processes in caspase-8 activation.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16632-42. doi: 
            10.1074/jbc.M109.095083. Epub 2010 Mar 22.
  PUBMED    20308068
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.095083"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16632-42"
COMMENT     Sequence Label: Fv-hCaspase 8 (C/A)-2A-GFP
FEATURES             Location/Qualifiers
     source          1..2016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..42
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             49..369
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1234..1287
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1297..1299,1300..1302,1303..2016)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1297..1299,1300..1302,1303..2013)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1342..1363)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1603..1622)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1950..1971
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggggagta gcaagagcaa gcctaaggac cccagccagc gctctagagg agtgcaggtg
       61 gagactatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
      121 cactacaccg ggatgcttga agatggaaag aaagttgatt cctcccggga cagaaacaag
      181 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
      241 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
      301 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
      361 aaactggaaa ctagtggagg tggaggttct agaggatccc atatgacaat ctcggactct
      421 ccaagagaac aggatagtga atcacagact ttggacaaag tttaccaaat gaaaagcaaa
      481 cctcggggat actgtctgat catcaacaat cacaattttg caaaagcacg ggagaaagtg
      541 cccaaacttc acagcattag ggacaggaat ggaacacact tggatgcagg ggctttgacc
      601 acgacctttg aagagcttca ttttgagatc aagccccacg atgactgcac agtagagcaa
      661 atctatgaga ttttgaaaat ctaccaactc atggaccaca gtaacatgga ctgcttcatc
      721 tgctgtatcc tctcccatgg agacaagggc atcatctatg gcactgatgg acaggaggcc
      781 cccatctatg agctgacatc tcagttcact ggtttgaagt gcccttccct tgctggaaaa
      841 cccaaagtgt tttttattca ggctgctcag ggggataact accagaaagg tatacctgtt
      901 gagactgatt cagaggagca accctattta gaaatggatt tatcatcacc tcaaacgaga
      961 tatatcccgg atgaggctga ctttctgctg gggatggcca ctgtgaataa ctgtgtttcc
     1021 taccgaaacc ctgcagaggg aacctggtac atccagtcac tttgccagag cctgagagag
     1081 cgatgccctc gaggcgatga tattctcacc atcctgactg aagtgaacta tgaagtaagc
     1141 aacaaggatg acaagaaaaa catggggaaa cagatgcctc agcctacttt cacactaaga
     1201 aaaaaacttg tcttcccttc tgatgcgatc gcagagggca gaggaagtct tctaacatgc
     1261 ggtgacgtgg aggagaatcc cggccctggc gcgcctatgg tgagcaaggg cgaggagctg
     1321 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1381 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1441 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1501 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1561 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1621 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1681 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1741 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1801 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1861 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1921 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1981 gggatcactc tcggcatgga cgagctgtac aagtaa
//
