LOCUS       sequence-157353-       13705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of Gata2-Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13705)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lazarus Gata 2 flag
FEATURES             Location/Qualifiers
     source          1..13705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1800..1823
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             1820..1828
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    join(3311..3885,3886..3888,3889..3897)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3478..3495)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3705..3724
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3898..3900,3901..3903,3904..4617)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3898..3900,3901..3903,3904..4614)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3943..3964)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4204..4223)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4551..4572
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4811..5280
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5462..5479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5633..6221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5713..5732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6344..6478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6368..6387)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6422..6441
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6708..7307)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6791..6811)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7288..7307
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7390..7889)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7438..7457)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7831..7854
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      9921..11710
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(12204..12995,12996..13064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12827..12846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13065..13169)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatttg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagtt gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgact
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatctaagta agtaggatcc atttaaattc gaattcatgg
     1801 attacaagga tgacgacgat aaggaggtgg cgcctgagca gcctcgctgg atggcgcacc
     1861 ccgccgtatt gaatgcgcag caccccgact cgcaccatcc gggcctggcg cataactaca
     1921 tggagccagc acagctgctg cctcccgacg aggtggatgt cttcttcaac catctcgact
     1981 cgcagggcaa cccttactac gccaacccgg cccacgcgcg cgcgcgcgtt tcctacagcc
     2041 cggcgcatgc ccgtctcacc ggaggccaga tgtgccgacc acacttgttg cacagcccag
     2101 gcttgccgtg gctggacggg ggcaaagcag ctctctctgc cgccgctgcc catcaccaca
     2161 gtccctggac cgtcagcccg ttctccaaga ccccgctgca cccctcagct gctggagcac
     2221 ccggagggcc tctgtctgtt tacccagggg ctgcgggtgg gagcggggga ggcagtggga
     2281 gctccgtggc ctccctcacc cccactgcag cccactcggg ctcccatctc ttcggcttcc
     2341 cacccacgcc acccaaagaa gtgtctccag accccagcac aacaggagct gcttccccgg
     2401 cctcttcttc tgcagggggt agtgtagccc ggggtgagga caaggatggc gtcaagtacc
     2461 aagtgtcact ctccgagagc atgaagatgg aaggcggcag tcccctgcgc ccgggcctag
     2521 ctaccatggg cacccagcct gcaacacacc acccgatacc cacctatccc tcctatgtgc
     2581 ccgccgcagc tcatgactat ggcagcagtc tcttccatcc aggaggcttc ctgggtggcc
     2641 ccgcctccag cttcacccct aagcagagaa gcaaggctcg ctcctgctca gaaggccggg
     2701 agtgtgtcaa ctgtggtgcc acagccaccc ctctctggcg acgagatggc acgggccact
     2761 acctgtgcaa tgcctgtggg ctctaccaca agatgaatgg acagaaccgg ccgctcatca
     2821 agcccaagcg gaggctgtct gctgccagaa gagcgggcac ctgttgtgca aattgtcaga
     2881 cgacaaccac caccttatgg cgccggaacg ccaacgggga ccctgtgtgc aacgcctgtg
     2941 gcctctacta caagctgcac aatgttaaca ggccactgac catgaagaag gaagggatcc
     3001 agacccggaa tcggaagatg tccagcaaat ccaagaagag caagaaaggg gctgaatgtt
     3061 tcgaggagct ctccaagtgc atgcaagaga agtcaccgcc cttcagtgcg gctgccctgg
     3121 ctggacacat ggcacctgtg ggacacctcc caccttttag tcactctgga cacatcctac
     3181 ccacgcccac gcctatccac ccttcctcca gtctctcttt tggccacccc cacccgtcca
     3241 gcatggtgac tgccatgggc tagctcgagg gccggcgcgc cgcggccgct acgtaaattc
     3301 cgcccccccc cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     3361 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3421 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3481 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3541 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     3601 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     3661 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     3721 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     3781 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     3841 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     3901 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3961 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4021 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4081 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     4141 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4201 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4261 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4321 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     4381 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4441 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4501 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4561 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     4621 ggccggccgc cagcacagtg gtcgacgata aaataaaaga ttttatttag tctccagaaa
     4681 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     4741 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca
     4801 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     4861 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     4921 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
     4981 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     5041 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     5101 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     5161 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     5221 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     5281 catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc
     5341 atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5401 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5461 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5521 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5581 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5641 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5701 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5761 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5821 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5881 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5941 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6001 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6061 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6121 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6181 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6301 tatcaaaaag gatcttcacc tagatccttt tgcggccgaa ttagatccag acatgataag
     6361 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6421 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     6481 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     6541 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag agtcggtggg
     6601 cctcgggggc gggtgcgggg tcggcggggc cgccccgggt ggcttcggtc ggagccatgg
     6661 ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc
     6721 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     6781 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     6841 accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     6901 ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg
     6961 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     7021 gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     7081 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     7141 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cgggtcgacg
     7201 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg
     7261 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatgga aggtcgtctc
     7321 cttgtgaggg gtcaggggcg tgggtcaggg gatggtggcg gcaccggtcg tggcggccga
     7381 cctgcaggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt
     7441 ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag
     7501 cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa
     7561 ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt
     7621 gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga
     7681 cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa
     7741 ggggccacca aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg
     7801 ccagaggcca cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga
     7861 ctgccttggg aaaagcgcct cccctacccg gtagaattaa ttctcatgtt tgacagctta
     7921 tcatcgataa gctgatcctc acaggccgca cccagctttt cttccgttgc cccagtagca
     7981 tctctgtctg gtgaccttga agaggaagag gaggggtccc gagaatcccc atccctaccg
     8041 tccagcaaaa agggggacga ggaatttgag gcctggcttg aggctcagga cgcaaatctt
     8101 gaggatgttc agcgggagtt ttccgggctg cgagtaattg gtgatgagga cgaggatggt
     8161 tcggaggatg gggaattttc agacctggat ctgtctgaca gcgaccatga aggggatgag
     8221 ggtggggggg ctgttggagg gggcaggagt ctgcactccc tgtattcact gagcgtcgtc
     8281 taataaagat gtctattgat ctcttttagt gtgaatcatg tctgacgagg ggccaggtac
     8341 aggacctgga aatggcctag gagagaaggg agacacatct ggaccagaag gctccggcgg
     8401 cagtggacct caaagaagag ggggtgataa ccatggacga ggacggggaa gaggacgagg
     8461 acgaggaggc ggaagaccag gagccccggg cggctcagga tcagggccaa gacatagaga
     8521 tggtgtccgg agaccccaaa aacgtccaag ttgcattggc tgcaaaggga cccacggtgg
     8581 aacaggagca ggagcaggag cgggaggggc aggagcagga ggggcaggag caggaggagg
     8641 ggcaggagca ggaggggcag gagcaggagg tggaggccgg ggtcgaggag gcagtggagg
     8701 ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc gccggggtag
     8761 aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag gtcgtggacg
     8821 tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt ctccaccgcg
     8881 caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg attattttga
     8941 ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag cgatagagca
     9001 gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc agggtgatgg
     9061 aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc
     9121 gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc acgtagaaag
     9181 gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta gtaagacctc
     9241 cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc ttacaccatt
     9301 gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc cgctaaggga
     9361 gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg aggttttgaa
     9421 ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata tcagggtgac
     9481 tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta tggtggaagg
     9541 ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg gagatgaggg
     9601 tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg
     9661 tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct gttggtgtat
     9721 ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat tgggcatacc
     9781 catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa aacattagcg
     9841 acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc ttaaattcac
     9901 ctaagaatgg gagcaaccag caggaaaagg acaagcagcg aaaattcacg cccccttggg
     9961 aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc atatgctgac
    10021 tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc atatactacc
    10081 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
    10141 cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
    10201 cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc atatgctacc
    10261 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccaga
    10321 tataaattag gatagcatat actaccctaa tctctattag gatagcatat gctacccgga
    10381 tacagattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
    10441 tatagattag gatagcctat gctacccaga tataaattag gatagcatat actacccaga
    10501 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
    10561 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccatggcaa
    10621 cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac cctgtgcttg
    10681 gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc gcccctatct
    10741 tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt tttgtgggca
    10801 agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac acccttattt
    10861 tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac tccacaattt
    10921 caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg agccccgttt
    10981 aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt ccactctctt
    11041 tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc ctgcctggga
    11101 cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc atagccataa
    11161 attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg atttctattg
    11221 ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag gggtttgtga
    11281 gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa attttattct
    11341 gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact gttcacaact
    11401 cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag attcaggaga
    11461 gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg gttcactacc
    11521 ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt gggagatatc
    11581 gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc ccgttgggta
    11641 acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg ggaagcatat
    11701 gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg ccctcttgag
    11761 ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag cctcccgtag
    11821 tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc ttcagccaag
    11881 agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct gctccaggat
    11941 gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca actacagtca
    12001 gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc ccagttggca
    12061 agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg ggcagagatg
    12121 tcgtagtcag gtttagttcg tccggggcgg ggatctggcc gcaaatcaat ctaaagtata
    12181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    12361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12481 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    12541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    12661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    12721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    12841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    13021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13141 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc accagctttg
    13201 ctcttaggag tttcctaata catcccaaac tcaaatatat aaagcatttg acttgttcta
    13261 tgccctaggg ggcgggggga agctaagcca gcttttttta acatttaaaa tgttaattcc
    13321 attttaaatg cacagatgtt tttatttcat aagggtttca atgtgcatga atgctgcaat
    13381 attcctgtta ccaaagctag tataaataaa aatagataaa cgtggaaatt acttagagtt
    13441 tctgtcatta acgtttcctt cctcagttga caacataaat gcgctgctga gaagccagtt
    13501 tgcatctgtc aggatcaatt tcccattatg ccagtcatat taattactag tcaattagtt
    13561 gatttttatt tttgacatat acatgtgaaa gaccccacct gtaggtttgg caagctagct
    13621 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga aaagttcaga
    13681 tcaaggtcag gaacagatgg aacag
//
