LOCUS       sequence-157357-       13577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of Gata3-Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13577)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lazarus Gata 3 flag
FEATURES             Location/Qualifiers
     source          1..13577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1800..1823
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             1820..1828
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    join(3183..3757,3758..3760,3761..3769)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3350..3367)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3577..3596
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3770..3772,3773..3775,3776..4489)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3770..3772,3773..3775,3776..4486)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3815..3836)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4076..4095)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4423..4444
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4683..5152
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5334..5351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5505..6093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5585..5604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6216..6350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6240..6259)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6294..6313
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6580..7179)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6663..6683)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7160..7179
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7262..7761)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7310..7329)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7703..7726
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      9793..11582
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(12076..12867,12868..12936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12699..12718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12937..13041)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatttg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagtt gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgact
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatctaagta agtaggatcc atttaaattc gaattcatgg
     1801 attacaagga tgacgacgat aaggaggtga ctgcggacca gccgcgctgg gtgagccacc
     1861 atcaccccgc ggtcctcaac ggtcagcacc cagacacgca ccacccgggc ctcggccatt
     1921 cgtacatgga agctcagtat ccgctgacgg aagaggtgga cgtacttttt aacatcgatg
     1981 gtcaaggcaa ccacgtcccg tcctactacg gaaactccgt cagggctacg gtgcagaggt
     2041 atcctccgac ccaccacggg agccaggtat gccgcccgcc tctgctgcac ggatctctgc
     2101 cctggctgga tggcggcaaa gccctgagca gccaccacac cgcctcgccc tggaacctca
     2161 gccccttctc caagacgtcc atccaccacg gctctccggg gcctctgtcc gtttaccctc
     2221 cggcttcatc ctcttctctg gcggccggcc actccagtcc tcatctcttc accttcccgc
     2281 ccaccccgcc gaaagacgtc tccccagacc cgtcgctgtc caccccggga tccgccgggt
     2341 cggccaggca agatgagaaa gagtgcctca agtatcaggt gcagctgcca gatagcatga
     2401 agctggagac gtctcactct cgaggcagca tgaccaccct gggtggggcc tcatcctcag
     2461 cccaccaccc cattaccacc tatccgccct atgtgcccga gtacagctct ggactcttcc
     2521 cacccagcag cctgctggga ggatccccta ccgggttcgg atgtaagtcg aggcccaagg
     2581 cacgatccag cacagaaggc agggagtgtg tgaactgcgg ggcaacctct accccactgt
     2641 ggcggcgaga tggtaccggg cactaccttt gcaatgcctg cggactctac cataaaatga
     2701 atgggcagaa ccggcccctt atcaagccca agcgaaggct gtcggcagca aggagagcag
     2761 ggacatcctg cgcgaactgt cagaccacca ccaccaccct ctggaggagg aacgctaatg
     2821 gggacccggt ctgcaatgcc tgtgggctgt actacaagct tcataatatt aacagacccc
     2881 tgactatgaa gaaagaaggc atccagaccc gaaaccggaa gatgtctagc aaatcgaaaa
     2941 agtgcaaaaa ggtgcatgac gcgctggagg acttccccaa gagcagctcc ttcaacccgg
     3001 ccgctctctc cagacacatg tcatccctga gccacatctc tcccttcagc cactccagcc
     3061 acatgctgac cacaccgacg cccatgcatc cgccctccgg cctctccttc ggacctcacc
     3121 acccttccag catggtcacc gccatgggtt agagcggccg ctacgtaaat tccgcccccc
     3181 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     3241 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3301 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3361 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3421 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3481 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3541 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     3601 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     3661 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     3721 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa
     3781 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3841 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3901 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3961 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4021 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4081 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4141 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4201 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4261 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4321 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4381 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4441 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccggcc
     4501 gccagcacag tggtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg
     4561 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     4621 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
     4681 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     4741 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     4801 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     4861 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     4921 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     4981 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     5041 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     5101 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc
     5161 atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc
     5221 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcattaatga
     5281 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5341 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5401 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6181 aggatcttca cctagatcct tttgcggccg aattagatcc agacatgata agatacattg
     6241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     6361 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     6421 aaaacctcta caaatgtggt atggctgatt atgatcctct agagtcggtg ggcctcgggg
     6481 gcgggtgcgg ggtcggcggg gccgccccgg gtggcttcgg tcggagccat ggggtcgtgc
     6541 gctcctttcg gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt
     6601 catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag
     6661 ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc
     6721 gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg
     6781 gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc
     6841 caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg
     6901 cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc
     6961 caccgcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag
     7021 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccgggtcga cggtgtggcg
     7081 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggcccggg ggacgtcgtc
     7141 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gaaggtcgtc tccttgtgag
     7201 gggtcagggg cgtgggtcag gggatggtgg cggcaccggt cgtggcggcc gacctgcagg
     7261 tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg
     7321 tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc
     7381 tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa
     7441 gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca
     7501 tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct
     7561 gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac
     7621 caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc
     7681 cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg
     7741 ggaaaagcgc ctcccctacc cggtagaatt aattctcatg tttgacagct tatcatcgat
     7801 aagctgatcc tcacaggccg cacccagctt ttcttccgtt gccccagtag catctctgtc
     7861 tggtgacctt gaagaggaag aggaggggtc ccgagaatcc ccatccctac cgtccagcaa
     7921 aaagggggac gaggaatttg aggcctggct tgaggctcag gacgcaaatc ttgaggatgt
     7981 tcagcgggag ttttccgggc tgcgagtaat tggtgatgag gacgaggatg gttcggagga
     8041 tggggaattt tcagacctgg atctgtctga cagcgaccat gaaggggatg agggtggggg
     8101 ggctgttgga gggggcagga gtctgcactc cctgtattca ctgagcgtcg tctaataaag
     8161 atgtctattg atctctttta gtgtgaatca tgtctgacga ggggccaggt acaggacctg
     8221 gaaatggcct aggagagaag ggagacacat ctggaccaga aggctccggc ggcagtggac
     8281 ctcaaagaag agggggtgat aaccatggac gaggacgggg aagaggacga ggacgaggag
     8341 gcggaagacc aggagccccg ggcggctcag gatcagggcc aagacataga gatggtgtcc
     8401 ggagacccca aaaacgtcca agttgcattg gctgcaaagg gacccacggt ggaacaggag
     8461 caggagcagg agcgggaggg gcaggagcag gaggggcagg agcaggagga ggggcaggag
     8521 caggaggggc aggagcagga ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc
     8581 gaggaggtag tggaggccgg ggtcgaggag gtagtggagg ccgccggggt agaggacgtg
     8641 aaagagccag ggggggaagt cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa
     8701 agaggcccag gagtcccagt agtcagtcat catcatccgg gtctccaccg cgcaggcccc
     8761 ctccaggtag aaggccattt ttccaccctg taggggaagc cgattatttt gaataccacc
     8821 aagaaggtgg cccagatggt gagcctgacg tgcccccggg agcgatagag cagggccccg
     8881 cagatgaccc aggagaaggc ccaagcactg gaccccgggg tcagggtgat ggaggcaggc
     8941 gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg
     9001 agaacattgc agaaggttta agagctctcc tggctaggag tcacgtagaa aggactaccg
     9061 acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg tagtaagacc tccctttaca
     9121 acctaaggcg aggaactgcc cttgctattc cacaatgtcg tcttacacca ttgagtcgtc
     9181 tcccctttgg aatggcccct ggacccggcc cacaacctgg cccgctaagg gagtccattg
     9241 tctgttattt catggtcttt ttacaaactc atatatttgc tgaggttttg aaggatgcga
     9301 ttaaggacct tgttatgaca aagcccgctc ctacctgcaa tatcagggtg actgtgtgca
     9361 gctttgacga tggagtagat ttgcctccct ggtttccacc tatggtggaa ggggctgccg
     9421 cggagggtga tgacggagat gacggagatg aaggaggtga tggagatgag ggtgaggaag
     9481 ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc
     9541 cccgcactaa agaataaatc cccagtagac atcatgcgtg ctgttggtgt atttctggcc
     9601 atctgtcttg tcaccatttt cgtcctccca acatggggca attgggcata cccatgttgt
     9661 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac
     9721 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat
     9781 gggagcaacc agcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
     9841 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
     9901 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
     9961 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
    10021 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
    10081 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta cccagatata
    10141 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt
    10201 aggatagcat atactaccct aatctctatt aggatagcat atgctacccg gatacagatt
    10261 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
    10321 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
    10381 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
    10441 aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc
    10501 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
    10561 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
    10621 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
    10681 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
    10741 aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa
    10801 agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg
    10861 ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg
    10921 ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt
    10981 aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt
    11041 gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat
    11101 attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt gagggttata
    11161 ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt
    11221 cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt
    11281 attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg
    11341 cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga
    11401 atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc
    11461 ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc
    11521 tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg
    11581 tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct
    11641 tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg
    11701 ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac
    11761 ataaaggcaa tgttgtgttg cagtccacag actgcaaagt ctgctccagg atgaaagcca
    11821 ctcagtgttg gcaaatgtgc acatccattt ataaggatgt caactacagt cagagaaccc
    11881 ctttgtgttt ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac
    11941 caaccaactg aagggattac atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc
    12001 aggtttagtt cgtccggggc ggggatctgg ccgcaaatca atctaaagta tatatgagta
    12061 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12121 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12181 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12241 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12301 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12361 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12421 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12481 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12541 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    12601 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12661 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12721 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12781 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12841 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12901 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12961 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13021 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccaccagctt tgctcttagg
    13081 agtttcctaa tacatcccaa actcaaatat ataaagcatt tgacttgttc tatgccctag
    13141 ggggcggggg gaagctaagc cagctttttt taacatttaa aatgttaatt ccattttaaa
    13201 tgcacagatg tttttatttc ataagggttt caatgtgcat gaatgctgca atattcctgt
    13261 taccaaagct agtataaata aaaatagata aacgtggaaa ttacttagag tttctgtcat
    13321 taacgtttcc ttcctcagtt gacaacataa atgcgctgct gagaagccag tttgcatctg
    13381 tcaggatcaa tttcccatta tgccagtcat attaattact agtcaattag ttgattttta
    13441 tttttgacat atacatgtga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
    13501 gccattttgc aaggcatgga aaaatacata actgagaata gaaaagttca gatcaaggtc
    13561 aggaacagat ggaacag
//
