LOCUS       sequence-157390-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan 
            Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Generoso WC, Gottardi M, Oreb M, Boles E
  TITLE     Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
  JOURNAL   J Microbiol Methods. 2016 Aug;127:203-5. doi: 
            10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
  PUBMED    27327211
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods."; date: "2016-08"; volume: "127:203-5. doi"; pages: " 
            10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17"
COMMENT     Sequence Label: pDuRCC-K
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(57..76)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
       61 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
      121 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
      181 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
      241 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc
      301 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
      361 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
      421 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
      481 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt
      541 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
      601 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
      661 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
      721 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
      781 gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg
      841 tcatttgtat agttttttta tattgtagtt gttctatttt
//
