LOCUS       sequence-157395-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a C-terminal macro domain and 6xHis tag for expression in 
            E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Wild R, Hothorn M
  TITLE     The macro domain as fusion tag for carrier-driven crystallization.
  JOURNAL   Protein Sci. 2016 Oct 24. doi: 10.1002/pro.3073.
  PUBMED    27774698
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2016 Oct 24. doi: 10.1002/pro.3073."
COMMENT     Sequence Label: pMH-macroHC
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagcttggc catttcctgc
       61 acatagatgc ctatactctc gctgtcaaaa agcacgaagt acaccgtttt gatggaagag
      121 gacattgtag acacgaagta actggagatg gccttcagaa tcagctgagc tgctgtctgc
      181 tttggaaaac cgttcctgcc gctgccgatg gatggaaatg caatggattt cagcttctta
      241 tcatcagcca gggccaagca gtttttcact gtcttttcca gaagttcttc acacttgtct
      301 gcaccccaaa ctggactatt acagtggatc acaaacttgg caggcaggcc atggcctgcg
      361 ctgacagcag ctccagctac ttccaagggc ccgttctttt tccggagttc caggacagct
      421 tccacaaact ccttgccacc tttcttctcc agcgtgtttc ctacttcacc accgatgtag
      481 aagtcagtgt ttgtcgggtg aacgacagca tcactgtcga tcgaggcaat gtcagcctgt
      541 acaacttgca acttctggcc aaggaagagg ctcttggtgg agaggactgt gaagccgtcg
      601 gatcccgcac ctgaagttct ggcaatatca tatagttggg aaattttaac gactagttcg
      661 tcatagtttt ccttgaaaaa tggtttagag tctaatctaa cttggaaaac tggtttcaag
      721 ataaaacctg tcttcttatc gtgtttcttg ataatctttt ggaaaccagt gtagtttagt
      781 ctcgaaaact ttgctagatc gtgaacatcg gcaatgatat cacttagttc ttcttccaga
      841 atctcaaaat ctagctgagt aggaggatta ttggagtcta acaaacgaac agtatg
//
