LOCUS       sequence-157399-        6537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a Myc tagged VSV-G protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-VSV-G(P127D)-Myc
FEATURES             Location/Qualifiers
     source          1..6537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     617..637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     663..687
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          778..1350
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             join(1436..1483,1484..2968)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPDQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     CDS             2969..2998
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3228..3247)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3293..3348
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3347..3366)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3706..3722)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3706..3722)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3719..3741)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3730..3746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3754..3760,3761..3778,3779..3784))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3799..3820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3937..3954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4108..4696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4188..4207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4867..5658,5659..5727))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5490..5509
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5728..5832)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5858..6313)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5995..6016)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6207..6226
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6440..6462
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6454..6471
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6455..6471
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6481..6500
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6481..6499
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
       61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg
      781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
      841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
      901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
      961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
     1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
     1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
     1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
     1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
     1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
     1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     1381 tctcatcatt ttggcaaaga attcctcgac ggatccctcg aggaattctg acactatgaa
     1441 gtgccttttg tacttagcct ttttattcat tggggtgaat tgcaagttca ccatagtttt
     1501 tccacacaac caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc
     1561 aagctcagat ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc
     1621 caagagtcac aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac
     1681 tacttgtgat ttccgctggt atggaccgaa gtatataaca cattccatcc gatccttcac
     1741 tccatctgta gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa
     1801 tccaggcttc cctgatcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat
     1861 tgtccaggtg actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc
     1921 acagttcatc aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac
     1981 ctggcattct gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat
     2041 caccttcttc tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag
     2101 aagtaactac tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca
     2161 ttggggagtc agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc
     2221 tgcagccaga ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc
     2281 agtggatgta agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga
     2341 aacctggagc aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc
     2401 tcctaaaaac ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt
     2461 tgagaccaga tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat
     2521 gatcagtgga actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt
     2581 ggaaattgga cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat
     2641 gattggacat ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga
     2701 acatcctcac attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg
     2761 tgatactggg ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa
     2821 aagctctatt gcctcttttt tctttatcat agggttaatc attggactat tcttggttct
     2881 ccgagttggt atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac
     2941 agacatagag atgaaccgac ttggaaagga acagaaactg attagcgaag aagatctgta
     3001 actcaaatcc tgcacaacag attcttcatg tttggaccaa atcaacttgt gataccatgc
     3061 tcaaagaggc ctcaattata tttgagtttt taatttttat gaaaaaaaaa aaaaaaaacg
     3121 gaattcctcg agggatccgt cgaggaattc actcctcagg tgcaggctgc ctatcagaag
     3181 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     3241 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     3301 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     3361 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     3421 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     3481 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     3541 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     3601 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     3661 ctcttatgga gatccctcga cggatcggcc gcaattcgta atcatgtcat agctgtttcc
     3721 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3781 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3841 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3901 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3961 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4021 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4081 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4141 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4201 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4261 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4321 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4381 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4441 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4501 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4561 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4621 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4681 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4741 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4801 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4861 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4921 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4981 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5041 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5101 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5161 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5221 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5281 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5341 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5401 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5461 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5521 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5581 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5641 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5701 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5761 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5821 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     5881 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     5941 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     6001 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     6061 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     6121 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     6181 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     6241 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     6301 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6361 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6421 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     6481 taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccg ctctaga
//
