LOCUS       sequence-157400-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of EPN-01 mutated in both L domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-01(Delta-L1+L2)
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             780..809
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacgggccct ctagaattat cgatccggag gtacccgggc ggccgcatgg
      121 gtgcgcgtgc gtctggttct aaatctggct ccggctctga ctctggtagc aaaatcgaag
      181 agctgttcaa gaaacacaag atcgttgccg tgctgcgtgc caatagtgtg gaagaagcga
      241 aaaagaaagc gctggcggtt ttcctgggcg gcgttcatct gattgaaatt acctttaccg
      301 tgccggatgc ggataccgtg attaaggaac tgagctttct gaaggaaatg ggcgcgatta
      361 ttggtgcggg caccgtgacc agcgtggagc agtgccgtaa agcggtggaa agtggcgccg
      421 aattcattgt gagtccgcac ctggacgagg aaattagcca attttgcaag gagaagggtg
      481 tgttctatat gccaggcgtt atgaccccga ccgaactggt gaaagccatg aaactgggcc
      541 ataccatctt aaaactgttt ccgggtgagg tggtgggtcc gcagtttgtt aaagcgatga
      601 aaggtccgtt tccgaatgtg aaatttgtgc caaccggcgg tgttaatctg gacaatgtgt
      661 gcgaatggtt caaagcgggc gtgctggccg tgggcgtggg cagcgcgtta gtgaaaggca
      721 ccccggtgga agtggcggaa aaggccaagg cgttcgttga gaagattcgt ggctgcaccg
      781 aacagaaact gattagcgaa gaagatctgc agtctcgtcc gga
//
