LOCUS       sequence-157401-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of EPN-01 mutated in both L domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-01(Delta-L1+L2)
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(220..249)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ggcttcatga tgtccccata atttttggca gagggaaaaa gatctgctag caagcttctc
       61 gagtcactga gaagacgggt cgttaccgaa cagagaacgc agagaggtca gcgcggccag
      121 ttctttgtcg atcggctcct gtttttgcgg tggcgtggtg gtttcaacac cagaacggaa
      181 agattcttcc ggcgctgcgg ccgcttccgg acgagactgc agatcttctt cgctaatcag
      241 tttctgttcg gtgcagccac gaatcttctc aacgaacgcc ttggcctttt ccgccacttc
      301 caccggggtg cctttcacta acgcgctgcc cacgcccacg gccagcacgc ccgctttgaa
      361 ccattcgcac acattgtcca gattaacacc gccggttggc acaaatttca cattcggaaa
      421 cggacctttc atcgctttaa caaactgcgg acccaccacc tcacccggaa acagttttaa
      481 gatggtatgg cccagtttca tggctttcac cagttcggtc ggggtcataa cgcctggcat
      541 atagaacaca cccttctcct tgcaaaattg gctaatttcc tcgtccaggt gcggactcac
      601 aatgaattcg gcgccacttt ccaccgcttt acggcactgc tccacgctgg tcacggtgcc
      661 cgcaccaata atcgcgccca tttccttcag aaagctcagt tccttaatca cggtatccgc
      721 atccggcacg gtaaaggtaa tttcaatcag atgaacgccg cccaggaaaa ccgccagcgc
      781 tttctttttc gcttcttcca cactattggc acgcagcacg gca
//
