LOCUS       sequence-157402-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of I3-01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-I3-01-Myc
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             644..673
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(698..717)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     primer_bind     complement(772..789)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 attatcgatc cggaggtacc cgggcggccg catgaaaatg gaagagctgt tcaagaaaca
       61 caagatcgtt gccgtgctgc gtgccaatag tgtggaagaa gcgaaaaaga aagcgctggc
      121 ggttttcctg ggcggcgttc atctgattga aattaccttt accgtgccgg atgcggatac
      181 cgtgattaag gaactgagct ttctgaagga aatgggcgcg attattggtg cgggcaccgt
      241 gaccagcgtg gagcagtgcc gtaaagcggt ggaaagtggc gccgaattca ttgtgagtcc
      301 gcacctggac gaggaaatta gccaattttg caaggagaag ggtgtgttct atatgccagg
      361 cgttatgacc ccgaccgaac tggtgaaagc catgaaactg ggccatacca tcttaaaact
      421 gtttccgggt gaggtggtgg gtccgcagtt tgttaaagcg atgaaaggtc cgtttccgaa
      481 tgtgaaattt gtgccaaccg gcggtgttaa tctggacaat gtgtgcgaat ggttcaaagc
      541 gggcgtgctg gccgtgggcg tgggcagcgc gttagtgaaa ggcaccccgg tggaagtggc
      601 ggaaaaggcc aaggcgttcg ttgagaagat tcgtggctgc accgaacaga aactgattag
      661 cgaagaagat ctgtaactcg agaagcttgc tagcagatct ttttccctct gccaaaaatt
      721 atggggacat catgaagccc cttgagcact taagtttaaa ccgctgatca gcctcgactg
      781 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      841 a
//
