LOCUS       sequence-157403-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of I3-01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-I3-01-Myc
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(70..99)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(781..800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(782..800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aggggcttca tgatgtcccc ataatttttg gcagagggaa aaagatctgc tagcaagctt
       61 ctcgagttac agatcttctt cgctaatcag tttctgttcg gtgcagccac gaatcttctc
      121 aacgaacgcc ttggcctttt ccgccacttc caccggggtg cctttcacta acgcgctgcc
      181 cacgcccacg gccagcacgc ccgctttgaa ccattcgcac acattgtcca gattaacacc
      241 gccggttggc acaaatttca cattcggaaa cggacctttc atcgctttaa caaactgcgg
      301 acccaccacc tcacccggaa acagttttaa gatggtatgg cccagtttca tggctttcac
      361 cagttcggtc ggggtcataa cgcctggcat atagaacaca cccttctcct tgcaaaattg
      421 gctaatttcc tcgtccaggt gcggactcac aatgaattcg gcgccacttt ccaccgcttt
      481 acggcactgc tccacgctgg tcacggtgcc cgcaccaata atcgcgccca tttccttcag
      541 aaagctcagt tccttaatca cggtatccgc atccggcacg gtaaaggtaa tttcaatcag
      601 atgaacgccg cccaggaaaa ccgccagcgc tttctttttc gcttcttcca cactattggc
      661 acgcagcacg gcaacgatct tgtgtttctt gaacagctct tccattttca tgcggccgcc
      721 cgggtacctc cggatcgata attctagagg gcccgtttaa acgctagcca gcttgggtct
      781 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
      841 ag
//
