LOCUS       sequence-157410-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector encoding full length UBR5 Y2402A/K2415A 
            (∆PABC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, 
            Stokke T, Patzke S, Saunders DN
  TITLE     The E3 ubiquitin ligase UBR5 regulates centriolar satellite 
            stability and primary cilia.
  JOURNAL   Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 
            10.1091/mbc.E17-04-0248.
  PUBMED    29742019
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248."
COMMENT     Sequence Label: pENTR221 UBR5 ∆PABC
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggcttccgga ccatgacgtc catccatttc
      121 gtggttcacc cgctgccggg caccgaggac cagctcaatg acaggttacg agaagtttct
      181 gagaagctga acaaatataa tttaaacagc cacccccctt tgaatgtatt ggaacaggct
      241 actattaaac agtgtgtggt gggaccaaat catgctgcct ttcttcttga ggatggtaga
      301 gtttgcagga ttggtttttc agtacagcca gacagattgg aattgggtaa acctgataat
      361 aatgatgggt caaagttgaa cagcaactcg ggggcaggga ggacgtcaag gcctggtagg
      421 acaagcgact ctccatggtt tctctcaggt tctgagactc taggcaggct ggcaggcaac
      481 accttaggaa gccgctggag ttctggagtg ggtggaagtg gtggaggatc ctctggtagg
      541 tcatcagctg gagctcgaga ttcccgccgg cagactcgag ttattcggac aggacgggat
      601 cgagggtctg ggcttttggg cagtcagccc cagccagtta ttccagcatc tgtcattcca
      661 gaggagctga tttcacaggc ccaagttgtt ttacaaggca aatccagaag tgtcattatt
      721 cgagaacttc agagaacaaa tcttgatgtg aaccttgctg taaataattt acttagccgg
      781 gatgatgaag atggagatga tggggatgat acagccagcg aatcttattt gcctggagag
      841 gatcttatgt ctctccttga tgccgacatt cattctgccc acccaagtgt cattattgat
      901 gcagatgcca tgttttctga agacattagc tattttggtt acccttcttt tcgtcgttca
      961 tcactttcca ggctaggctc atctcgagtt ctccttcttc ccttagagag agactctgag
     1021 ctgttgcgtg aacgtgaatc cgttttacgt t
//
