LOCUS       sequence-157414-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCYT2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Chidley C, Trauger SA, Birsoy K, O'Shea EK
  TITLE     The anticancer natural product ophiobolin A induces cytotoxicity by 
            covalent modification of phosphatidylethanolamine.
  JOURNAL   Elife. 2016 Jul 12;5. pii: e14601. doi: 10.7554/eLife.14601.
  PUBMED    27403889
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-07-12"; volume: "5. pii: e14601. doi"; pages: " 
            10.7554/eLife.14601"
COMMENT     Sequence Label: pLJM1-PCYT2
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(3..513)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(66..84)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    562..679
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccctggggag agaggtcggt gattcggtca acgagggagc cgactgccga cgtgcgctcc
       61 ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca gggcccacac taccgcccca
      121 caccccgcct cccgcaccgc cccttcccgg ccgctgctct cggcgcgccc tgctgagcag
      181 ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca ttgctccctg gcgctgtccg
      241 tctgcgaggg tactagtgag acgtgcggct tccgtttgtc acgtccggca cgccgcgaac
      301 cgcaaggaac cttcccgact taggggcgga gcaggaagcg tcgccggggg gcccacaagg
      361 gtagcggcga agatccgggt gacgctgcga acggacgtga agaatgtgcg agacccaggg
      421 tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc cgcgtccctg cgcaaaccca
      481 gggctgcctt ggaaaaggcg caaccccaac cccctcgagc cgcggccaaa gtggatctct
      541 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
      601 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
      661 cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctcgagg tcgagaattc
      721 gaagatttag aagtcaccat cgcgctcccc caggggctgt gccgcctgct gcctggcagc
      781 ctccaggaag gccagctcct tggcttcctt cttctggttt cgcgcctcat actccaacct
      841 gttggtgatg atccgctgga cgatgaggtc tgtggtgagg ttgctgccac tgtcaatctg
      901 acggaagatg ccccttctct tgggctcctg gtatgggtcg gagccatccc tgtcagggat
      961 aatttctgtc ttgccgtgac acaccaggtc caccttgaag tgactta
//
