LOCUS       sequence-157415-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a
            Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA 
            tail, followed by FRT site-flanked Kanamycin selection gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..57
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             64..90
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             139..159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggccgccat ggcggccgcg gatggccacc tatgacgtcc cagattacgc aagtttgccc
       61 ggatatgatg ttcctgatta tgctagcctc ccgggttacg atgtgcccga ctatgcctca
      121 cttccaggca gatctcctcc aaaaaagaag agaaaagtac gatctatgaa ggataacacc
      181 gtgccactga aattgattgc cctgttagcg aacggtgaat ttcactctgg cgagcagttg
      241 ggtgaaacgc tgggaatgag ccgggcggct attaataaac acattcagac actgcgtgac
      301 tggggcgttg atgtctttac cgttccggga aaaggataca gcctgcctga gcctatccag
      361 ttacttaatg ctaaacagat attgggtcag ctggatggcg gtagtgtagc cgtgctgcca
      421 gtgattgact ccacgaatca gtaccttctt gatcgtatcg gagagcttaa atcgggcgat
      481 gcttgcattg cagaatacca gcaggctggc cgtggtcgcc ggggtcggaa atggttttcg
      541 ccttttggcg caaacttata tttgtcgatg ttctggcgtc tggaacaagg cccggcggcg
      601 gcgattggtt taagtctggt tatcggtatc gtgatggcgg aagtattacg caagctgggt
      661 gcagataaag ttcgtgttaa atggcctaat gacctctatc tgcaggatcg caagctggca
      721 ggcattctgg tggagctgac tggcaaaact ggcgatgcgg cgcaaatagt cattggagcc
      781 gggatcaaca tggcaatgcg ccgtgttgaa gagagtgtcg ttaatcaggg gtggatcacg
      841 ctgcaggaag cggggatcaa tctcgatcgt aatacgttgg cggccatgct aatacgtgaa
      901 ttacgtgctg cgttggaact cttcgaacaa gaaggattgg cac
//
