LOCUS       sequence-157416-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a
            Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA 
            tail, followed by FRT site-flanked Kanamycin selection gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..754)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(322..341)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(761..814)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gctcgaagcg gccggccgcc ccgactctag agtcgcggcc gctttacttg tacagctcgt
       61 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
      121 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
      181 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
      241 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
      301 aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
      361 tgccgcgcag cttcacttta taaatgaact cgccatcttg gagggaggag tcctgggtca
      421 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
      481 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
      541 acatgaattg aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcactt
      601 taagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
      661 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
      721 tggccatgtt atcctcctcg cccttcgaca ccatacttcc gggcccggga ttttcctcca
      781 cgtccccgca tgttagaaga cttcccctgc cctctcctga tccgatatct ttttctgcac
      841 tacgcaggga tatttcaccg cccatccagg gttttattat tccatcctgc tcaagtaata
      901 aagccccctg tttgtctatt ccgcgtgaaa tgccaaatat ttctttatca ccaatgata
//
