LOCUS       sequence-157417-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and membrane-tethered mCherry protein 
            (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-flk1-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(6..17,18..205)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt
       61 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa
      121 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa
      181 agtacttatt ttttggagat cacttgggcc cggctcgagg ttgtatgaag tttctgtgtg
      241 actggaaaaa aaccaacgct gcccctcgcg accgctcccc gccaaaacac gcacgaggac
      301 gcgcttcagc cggagtgtaa taccgctgag tgaccgcaag gtggtgtctc acacacggtt
      361 tttaacttgc tgttactcat catgtctccc taaaatattt cattcaggtt cacacaattt
      421 atttggcgtg gtgaagccaa gattaataat ccagcctcat taatattaac aactacaatc
      481 attttgctat ttggacatca ctcatatgct ataaaattgc atgtgtctgt atataataac
      541 tgctatttga ttgtaactta agtaactgag gtttacttaa agtaatgcaa ttatgtgatt
      601 gtaatgcaaa aatgtgcatc ttttgtaaag ctgcattgaa acaataatta ctgtaaaaag
      661 ctctgtacaa ataaacttaa atttaatatt tagttacatt atagcgcaca atttccacta
      721 tattaaatgc acttttatta taaatagtaa tagaatataa aaatgaaata taaaagcctc
      781 agaaacaagc gctctaaata atattttaag ataaatggag aactaggcct gctcttttga
      841 tattcagaat ttccgtgttg aaaggtctgg cc
//
