LOCUS       sequence-157421-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-MCS-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(6..17,18..205)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        289..307
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     289..306
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      369..378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             384..410
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             417..443
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             450..476
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt
       61 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa
      121 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa
      181 agtacttatt ttttggagat cacttgggcc cggctcgagc tacgtactta agcttgagga
      241 tcccatcgat tcgaattctc ggtacctagt cgaccttcgc gaaggcctat ttaggtgaca
      301 ctatagaaga gtacaattca aggttaagcg gccgcaagct agattggcat tccggtactg
      361 ttggtaaagc caccatggcc acctatgacg tcccagatta cgcaagtttg cccgggtatg
      421 atgttcctga ttatgctagc ctcccgggtt acgatgtgcc cgactatgcc tcacttccag
      481 gcagatctcc tatgaaggat aacaccgtgc cactgaaatt gattgccctg ttagcgaacg
      541 gtgaatttca ctctggcgag cagttgggtg aaacgctggg aatgagccgg gcggctatta
      601 ataaacacat tcagacactg cgtgactggg gcgttgatgt ctttaccgtt ccgggtaaag
      661 gatacagcct gcctgagcct atccagttac ttaatgctaa acagatattg ggtcagctgg
      721 atggcggtag tgtagccgtg ctgccagtga ttgactccac gaatcagtac cttcttgatc
      781 gtatcggaga gcttaaatcg ggcgatgctt gcattgcaga ataccagcag gctggccgtg
      841 gtcgccgggg tcggaaatgg ttttcgcctt ttggcgcaaa cttatatttg tcgatgttct
      901 ggcgtctgga acaaggcccg gcggcggcga ttggtttaag tctggttatc ggtatcg
//
