LOCUS       sequence-157423-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) 
            with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered 
            mCherry protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-MCS-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(8..19,20..207)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        291..309
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     291..308
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      371..380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             386..412
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             419..445
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             452..478
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             494..514
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caagaaccag aggtgtaaag tacttgagta attttacttg attactgtac ttaagtatta
       61 tttttgggga tttttacttt acttgagtac aattaaaaat caatactttt acttttactt
      121 aattacattt ttttagaaaa aaaagtactt tttactcctt acaattttat ttacagtcaa
      181 aaagtactta ttttttggag atcacttggg cccggctcga gctacgtact taagcttgag
      241 gatcccatcg attcgaattc tcggtaccta gtcgaccttc gcgaaggcct atttaggtga
      301 cactatagaa gagtacaatt caaggttaag cggccgcaag ctagattggc attccggtac
      361 tgttggtaaa gccaccatgg ccacctatga cgtcccagat tacgcaagtt tgcccgggta
      421 tgatgttcct gattatgcta gcctcccggg ttacgatgtg cccgactatg cctcacttcc
      481 aggcagatct cctccaaaaa agaagagaaa ggtacgatct atgaaggata acaccgtgcc
      541 actgaaattg attgccctgt tagcgaacgg tgaatttcac tctggcgagc agttgggtga
      601 aacgctggga atgagccggg cggctattaa taaacacatt cagacactgc gtgactgggg
      661 cgttgatgtc tttaccgttc cgggtaaagg atacagcctg cctgagccta tccagttact
      721 taatgctaaa cagatattgg gtcagctgga tggcggtagt gtagccgtgc tgccagtgat
      781 tgactccacg aatcagtacc ttcttgatcg tatcggagag cttaaatcgg gcgatgcttg
      841 cattgcagaa taccagcagg ctggccgtgg tcgccggggt cggaaatggt tttcgccttt
      901 tggcgca
//
