LOCUS       sequence-157427-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-myl7-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(6..17,18..205)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt
       61 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa
      121 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa
      181 agtacttatt ttttggagat cacttgggcc cggctcgagt gtgaccaaag cttaaatcag
      241 ttgtgttaaa taagagacat tcaaaataaa tgtaaatgag ctctccaaat cagcagactt
      301 aacattcttt aaaatgattg attcaatagt gatacaaatc aggcatagca gttgtaactt
      361 ttgataaatt acagaaaatg tcaaatacag agaaccgatt cttttttatg acacatccaa
      421 gcacacattt aacacaatcc aggcaaaccc cgaatttcag agtcacaagc actgtttgta
      481 caagagcttt gcctaaggac gcacagtctc tatgagtcca ggtcgttggt ttcactctta
      541 ttttaaacat gtgacatttt tcctgccatc ctgtcttagg ctgctgtttg cttcatttca
      601 tgtcacatta atttcctcag tagcaccttt tacacacaca gccaatcttt tccagaacat
      661 tcaattgctt tgaagagata atgtgtgaac aaatccattt agaaaaggaa aattaagaat
      721 ttgtaaaatc atctgtaaat tgttggcatt cttctgtata tgaacatcac atcatttaca
      781 ggtaaaggtc tggtcattaa ttatatgaca atttactggt attattttgt gaaaggggct
      841 attttcaatg cattcatcca tccttttcat ccctcaaatc tctcattcac gtccccctcc
      901 ccatctgcac actttatctc attttccacc ctgctggaat ctgagcactt gtgcagttat
      961 cagggctcct gtatttagga ggctct
//
