LOCUS       sequence-157431-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and membrane-tethered mCherry protein 
            (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-sox10-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..57
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             64..90
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gacgtcccag attacgcaag tttgcccggg tatgatgttc ctgattatgc tagcctcccg
       61 ggttacgatg tgcccgacta tgcctcactt ccaggcagat ctcctatgaa ggataacacc
      121 gtgccactga aattgattgc cctgttagcg aacggtgaat ttcactctgg cgagcagttg
      181 ggtgaaacgc tgggaatgag ccgggcggct attaataaac acattcagac actgcgtgac
      241 tggggcgttg atgtctttac cgttccgggt aaaggataca gcctgcctga gcctatccag
      301 ttacttaatg ctaaacagat attgggtcag ctggatggcg gtagtgtagc cgtgctgcca
      361 gtgattgact ccacgaatca gtaccttctt gatcgtatcg gagagcttaa atcgggcgat
      421 gcttgcattg cagaatacca gcaggctggc cgtggtcgcc ggggtcggaa atggttttcg
      481 ccttttggcg caaacttata tttgtcgatg ttctggcgtc tggaacaagg cccggcggcg
      541 gcgattggtt taagtctggt tatcggtatc gtgatggcgg aagtattacg caagctgggt
      601 gcagataaag ttcgtgttaa atggcctaat gacctctatc tgcaggatcg caagctggca
      661 ggcattctgg tggagctgac tggcaaaact ggcgatgcgg cgcaaatagt cattggagcc
      721 gggatcaaca tggcaatgcg ccgtgttgaa gagagtgtcg ttaatcaggg gtggatcacg
      781 ctgcaggaag cggggatcaa tctcgatcgt aatacgttgg cggccatgct aatacgtgaa
      841 ttacgtgctg cgttggaact cttcgaacaa gaaggattgg caccttatct gtcgcgctgg
      901 gaaaagctgg ataattttat taatcgccca gtgaaactta tcattggtga taaagaaata
      961 tttggcattt cacgcggaat agacaa
//
