LOCUS       sequence-157433-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-sox10-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(8..19,20..207)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 caagaaccag aggtgtaaag tacttgagta attttacttg attactgtac ttaagtatta
       61 tttttgggga tttttacttt acttgagtac aattaaaaat caatactttt acttttactt
      121 aattacattt ttttagaaaa aaaagtactt tttactcctt acaattttat ttacagtcaa
      181 aaagtactta ttttttggag atcacttggg cccggctcga gctacgtact taagcttgag
      241 gatcccatcg attcgaattc ctgcagcccg ggggatcccc ttatcagagt caacattcat
      301 ggataatgaa aaaggcaatg atttttggct tgggtggggg ggccgtcgtg agatggaaat
      361 acaagaggtt attgttgacc agaacagcgc gcgtgtttgt gtgcgtttgg cccgtcgttt
      421 gcctctcttc tcgtctttct atattttcta ctaaagctgt ttaaacgcgt taaatgcgct
      481 ctctgtgtgt gtttatgcgc gctgatgcaa ttataacact aacacacata gcgcgacacg
      541 atgcaggagt tcattccagt gctgcgggcc tgcgcgcgca gcatgaacac accactgcga
      601 gcaaatctgc aatattaatg aatatatata actaaagtgt gcactttctg gtttataact
      661 gcagcacccc gcatacacca cagtataaag taatctacaa tattaatgaa tataataaac
      721 aagaatgttc acttttaggt ttaagaatgc aggcctgcgc gcgcagcatc ccgcgagcac
      781 aacactagaa actaaactcc aatattagtt aatatattca attaaaatgt acactttctg
      841 gtttaaaact gcaagtatgc gtgcgcagca tccgacatac ccaacactag aaactaatct
      901 acaatattta tgaaagtatt caactaaaat gttc
//
