LOCUS       sequence-157434-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-sox10-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..55
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             62..88
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             104..124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgtcccagat tacgcaagtt tgcccgggta tgatgttcct gattatgcta gcctcccggg
       61 ttacgatgtg cccgactatg cctcacttcc aggcagatct cctccaaaaa agaagagaaa
      121 ggtacgatct atgaaggata acaccgtgcc actgaaattg attgccctgt tagcgaacgg
      181 tgaatttcac tctggcgagc agttgggtga aacgctggga atgagccggg cggctattaa
      241 taaacacatt cagacactgc gtgactgggg cgttgatgtc tttaccgttc cgggtaaagg
      301 atacagcctg cctgagccta tccagttact taatgctaaa cagatattgg gtcagctgga
      361 tggcggtagt gtagccgtgc tgccagtgat tgactccacg aatcagtacc ttcttgatcg
      421 tatcggagag cttaaatcgg gcgatgcttg cattgcagaa taccagcagg ctggccgtgg
      481 tcgccggggt cggaaatggt tttcgccttt tggcgcaaac ttatatttgt cgatgttctg
      541 gcgtctggaa caaggcccgg cggcggcgat tggtttaagt ctggttatcg gtatcgtgat
      601 ggcggaagta ttacgcaagc tgggtgcaga taaagttcgt gttaaatggc ctaatgacct
      661 ctatctgcag gatcgcaagc tggcaggcat tctggtggag ctgactggca aaactggcga
      721 tgcggcgcaa atagtcattg gagccgggat caacatggca atgcgccgtg ttgaagagag
      781 tgtcgttaat caggggtgga tcacgctgca ggaagcgggg atcaatctcg atcgtaatac
      841 gttggcggcc atgctaatac gtgaattacg tgctgcgttg gaactcttcg aacaagaagg
      901 attggcacct tatctgtcgc gctgggaaaa gctggataat tttattaatc gcccagtgaa
      961 acttatcatt ggtgataaag aaatatttgg catttcac
//
