LOCUS       sequence-157439-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and membrane-tethered mCherry protein 
            (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-zic2a-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             111..137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             144..170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 tcgattcgaa ttcaaggtta agcggccgca agctagattg gcattccggt actgttggta
       61 aagccaccat ggccacctat gacgtcccag attacgcaag tttgcccggg tatgatgttc
      121 ctgattatgc tagcctcccg ggttacgatg tgcccgacta tgcctcactt ccaggcagat
      181 ctcctatgaa ggataacacc gtgccactga aattgattgc cctgttagcg aacggtgaat
      241 ttcactctgg cgagcagttg ggtgaaacgc tgggaatgag ccgggcggct attaataaac
      301 acattcagac actgcgtgac tggggcgttg atgtctttac cgttccgggt aaaggataca
      361 gcctgcctga gcctatccag ttacttaatg ctaaacagat attgggtcag ctggatggcg
      421 gtagtgtagc cgtgctgcca gtgattgact ccacgaatca gtaccttctt gatcgtatcg
      481 gagagcttaa atcgggcgat gcttgcattg cagaatacca gcaggctggc cgtggtcgcc
      541 ggggtcggaa atggttttcg ccttttggcg caaacttata tttgtcgatg ttctggcgtc
      601 tggaacaagg cccggcggcg gcgattggtt taagtctggt tatcggtatc gtgatggcgg
      661 aagtattacg caagctgggt gcagataaag ttcgtgttaa atggcctaat gacctctatc
      721 tgcaggatcg caagctggca ggcattctgg tggagctgac tggcaaaact ggcgatgcgg
      781 cgcaaatagt cattggagcc gggatcaaca tggcaatgcg ccgtgttgaa gagagtgtcg
      841 ttaatcaggg gtggatcacg ctgcaggaag cggggatcaa tctcgatcgt aatacgttgg
      901 cggccatgct aatacgtgaa ttacgtgctg cgttggaact cttcgaa
//
