LOCUS       sequence-157440-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-zic2a-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(6..17,18..205)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    225..249
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt
       61 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa
      121 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa
      181 agtacttatt ttttggagat cacttgggcc cggctcgaga tatcaccact ttgtacaaga
      241 aagctgggtc actggaccca catattcctc gtgttccagt gccagtgtac ctgaacatta
      301 acaatacccc tgtctcgcct ccccttcttg tctttttcac ccctttctgg tctcattact
      361 ttgggtttta tgccctaatc tacacaatga ggacgaagtc atggtggtta tcttcagcag
      421 ccaatgcctg cctacctgta atccttccca aagtccctgg ctttctcaca gctcacaaat
      481 cggcacacaa tgctttcatg caaggcctct gccgtaatgc tcaaagacga cagcagattt
      541 ttttcttttc tcatcccttc acatcaaatc ccacccttcc tctgaaaaac agggacgtct
      601 tgatctaagt ctcaggacaa ggaaatgtta gaatataaag gacacaagag ataaatgtaa
      661 gtttctccag atacctcagc acctctgtta cataaacgga agtaaagtaa aactcctgct
      721 gcactgacac tgttatatat atgagcagaa tgatacggtt ggttataaat gacacttaaa
      781 aaaatcaatg aatttatcat taaaagttaa ttgagaaaga gctcaaaaag gtcaagccag
      841 ttgctccatt tgaggagggc ataaaggggt taggttttaa ctt
//
