LOCUS       sequence-157441-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-zic2a-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             111..137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             144..170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             186..206
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgattcgaa ttcaaggtta agcggccgca agctagattg gcattccggt actgttggta
       61 aagccaccat ggccacctat gacgtcccag attacgcaag tttgcccggg tatgatgttc
      121 ctgattatgc tagcctcccg ggttacgatg tgcccgacta tgcctcactt ccaggcagat
      181 ctcctccaaa aaagaagaga aaggtacgat ctatgaagga taacaccgtg ccactgaaat
      241 tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt gaaacgctgg
      301 gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg ggcgttgatg
      361 tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc tatccagtta cttaatgcta
      421 aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtg attgactcca
      481 cgaatcagta ccttcttgat cgtatcggag agcttaaatc gggcgatgct tgcattgcag
      541 aataccagca ggctggccgt ggtcgccggg gtcggaaatg gttttcgcct tttggcgcaa
      601 acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg attggtttaa
      661 gtctggttat cggtatcgtg atggcggaag tattacgcaa gctgggtgca gataaagttc
      721 gtgttaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc attctggtgg
      781 agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg atcaacatgg
      841 caatgcgccg tgttgaagag agtgtcgtta atcaggggtg gatcacgctg caggaagcgg
      901 ggatcaatct cgatcgtaat acgttggcgg ccatgctaat acgtgaatta cgtgctgcgt
      961 tggaactctt cgaacaagaa ggattggcac cttatctgtc gcgctgggaa aagctggata
     1021 attttattaa
//
