LOCUS       sequence-157442-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             121..147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             154..180
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 caggatccca tcgattcgaa ttcaaggtta agcggccgca agctagattg gcattccggt
       61 actgttggta aagccaccat ggccacctat gacgtcccag attacgcaag tttgcccggg
      121 tatgatgttc ctgattatgc tagcctcccg ggttacgatg tgcccgacta tgcctcactt
      181 ccaggcagat ctcctatgaa ggataacacc gtgccactga aattgattgc cctgttagcg
      241 aacggtgaat ttcactctgg cgagcagttg ggtgaaacgc tgggaatgag ccgggcggct
      301 attaataaac acattcagac actgcgtgac tggggcgttg atgtctttac cgttccgggt
      361 aaaggataca gcctgcctga gcctatccag ttacttaatg ctaaacagat attgggtcag
      421 ctggatggcg gtagtgtagc cgtgctgcca gtgattgact ccacgaatca gtaccttctt
      481 gatcgtatcg gagagcttaa atcgggcgat gcttgcattg cagaatacca gcaggctggc
      541 cgtggtcgcc ggggtcggaa atggttttcg ccttttggcg caaacttata tttgtcgatg
      601 ttctggcgtc tggaacaagg cccggcggcg gcgattggtt taagtctggt tatcggtatc
      661 gtgatggcgg aagtattacg caagctgggt gcagataaag ttcgtgttaa atggcctaat
      721 gacctctatc tgcaggatcg caagctggca ggcattctgg tggagctgac tggcaaaact
      781 ggcgatgcgg cgcaaatagt cattggagcc gggatcaaca tggcaatgcg ccgtgttgaa
      841 gagagtgtcg ttaatcaggg gtggatcacg ctgcaggaag cggggatcaa tctcgatcgt
      901 aatacgttgg cggccatgct aatacgtgaa ttacgtgctg cgttgga
//
