LOCUS       sequence-157443-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(140..847)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(415..434)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     684..702
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(854..907)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cggttaatac gactcactat agggagtccg gatacgtaat acgactcact atagttctag
       61 atcaggagag cacacacttg cagctcatgc agccggggcc actctcatca ggagggttca
      121 gcttagatct tcctcctccc ttgtacagct cgtccatgcc gccggtggag tggcggccct
      181 cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact
      241 tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct
      301 tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct
      361 tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct
      421 tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga
      481 actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt
      541 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt
      601 cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc
      661 aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
      721 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca
      781 tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc
      841 tcaccataga tctgggcccg ggattttcct ccacgtcccc gcatgttaga agacttcccc
      901 tgccctctcc tgatccgata tctttttctg cactacgcag ggatatttca ccgcccatcc
      961 agggttttat tattccatcc tgctcaagta ataaagcccc ctgtttgtct attccgcgtg
     1021 aaatgccaaa tatttcttta tca
//
