LOCUS       sequence-157445-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with 
            NLS, a Thosea asigna virus peptide (2A), and membrane-tethered 
            mCherry protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             121..147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             154..180
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             196..216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caggatccca tcgattcgaa ttcaaggtta agcggccgca agctagattg gcattccggt
       61 actgttggta aagccaccat ggccacctat gacgtcccag attacgcaag tttgcccggg
      121 tatgatgttc ctgattatgc tagcctcccg ggttacgatg tgcccgacta tgcctcactt
      181 ccaggcagat ctcctccaaa aaagaagaga aaggtacgat ctatgaagga taacaccgtg
      241 ccactgaaat tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt
      301 gaaacgctgg gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg
      361 ggcgttgatg tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc tatccagtta
      421 cttaatgcta aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtg
      481 attgactcca cgaatcagta ccttcttgat cgtatcggag agcttaaatc gggcgatgct
      541 tgcattgcag aataccagca ggctggccgt ggtcgccggg gtcggaaatg gttttcgcct
      601 tttggcgcaa acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg
      661 attggtttaa gtctggttat cggtatcgtg atggcggaag tattacgcaa gctgggtgca
      721 gataaagttc gtgttaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc
      781 attctggtgg agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg
      841 atcaacatgg caatgcgccg tgttgaagag agtgtcgtta atcagggg
//
