LOCUS       sequence-157570-        3207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CA, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_PIK3CA_p.E453Q
FEATURES             Location/Qualifiers
     source          1..3207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcctccac gaccatcatc aggtgaactg tggggcatcc acttgatgcc cccaagaatc
       61 ctagtagaat gtttactacc aaatggaatg atagtgactt tagaatgcct ccgtgaggct
      121 acattaataa ccataaagca tgaactattt aaagaagcaa gaaaataccc cctccatcaa
      181 cttcttcaag atgaatcttc ttacattttc gtaagtgtta ctcaagaagc agaaagggaa
      241 gaattttttg atgaaacaag acgactttgt gaccttcggc tttttcaacc ctttttaaaa
      301 gtaattgaac cagtaggcaa ccgtgaagaa aagatcctca atcgagaaat tggttttgct
      361 atcggcatgc cagtgtgtga atttgatatg gttaaagatc cagaagtaca ggacttccga
      421 agaaatattc tgaacgtttg taaagaagct gtggatctta gggacctcaa ttcacctcat
      481 agtagagcaa tgtatgtcta tcctccaaat gtagaatctt caccagaatt gccaaagcac
      541 atatataata aattagataa agggcaaata atagtggtga tctgggtaat agtttctcca
      601 aataatgaca agcagaagta tactctgaaa atcaaccatg actgtgtacc agaacaagta
      661 attgctgaag caatcaggaa aaaaactcga agtatgttgc tatcctctga acaactaaaa
      721 ctctgtgttt tagaatatca gggcaagtat attttaaaag tgtgtggatg tgatgaatac
      781 ttcctagaaa aatatcctct gagtcagtat aagtatataa gaagctgtat aatgcttggg
      841 aggatgccca atttgatgtt gatggctaaa gaaagccttt attctcaact gccaatggac
      901 tgttttacaa tgccatctta ttccagacgc atttccacag ctacaccata tatgaatgga
      961 gaaacatcta caaaatccct ttgggttata aatagtgcac tcagaataaa aattctttgt
     1021 gcaacctacg tgaatgtaaa tattcgagac attgataaga tctatgttcg aacaggtatc
     1081 taccatggag gagaaccctt atgtgacaat gtgaacactc aaagagtacc ttgttccaat
     1141 cccaggtgga atgaatggct gaattatgat atatacattc ctgatcttcc tcgtgctgct
     1201 cgactttgcc tttccatttg ctctgttaaa ggccgaaagg gtgctaaaga ggaacactgt
     1261 ccattggcat ggggaaatat aaacttgttt gattacacag acactctagt atctggaaaa
     1321 atggctttga atctttggcc agtacctcat ggattacaag atttgctgaa ccctattggt
     1381 gttactggat caaatccaaa taaagaaact ccatgcttag agttggagtt tgactggttc
     1441 agcagtgtgg taaagttccc agatatgtca gtgattgaag agcatgccaa ttggtctgta
     1501 tcccgagaag caggatttag ctattcccac gcaggactga gtaacagact agctagagac
     1561 aatgaattaa gggaaaatga caaagaacag ctcaaagcaa tttctacacg agatcctctc
     1621 tctgaaatca ctgagcagga gaaagatttt ctatggagtc acagacacta ttgtgtaact
     1681 atccccgaaa ttctacccaa attgcttctg tctgttaaat ggaattctag agatgaagta
     1741 gcccagatgt attgcttggt aaaagattgg cctccaatca aacctgaaca ggctatggaa
     1801 cttctggact gtaattaccc agatcctatg gttcgaggtt ttgctgttcg gtgcttggaa
     1861 aaatatttaa cagatgacaa actttctcag tatttaattc agctagtaca ggtcctaaaa
     1921 tatgaacaat atttggataa cttgcttgtg agatttttac tgaagaaagc attgactaat
     1981 caaaggattg ggcacttttt cttttggcat ttaaaatctg agatgcacaa taaaacagtt
     2041 agccagaggt ttggcctgct tttggagtcc tattgtcgtg catgtgggat gtatttgaag
     2101 cacctgaata ggcaagtcga ggcaatggaa aagctcatta acttaactga cattctcaaa
     2161 caggagaaga aggatgaaac acaaaaggta cagatgaagt ttttagttga gcaaatgagg
     2221 cgaccagatt tcatggatgc tctacagggc tttctgtctc ctctaaaccc tgctcatcaa
     2281 ctaggaaacc tcaggcttga agagtgtcga attatgtcct ctgcaaaaag gccactgtgg
     2341 ttgaattggg agaacccaga catcatgtca gagttactgt ttcagaacaa tgagatcatc
     2401 tttaaaaatg gggatgattt acggcaagat atgctaacac ttcaaattat tcgtattatg
     2461 gaaaatatct ggcaaaatca aggtcttgat cttcgaatgt taccttatgg ttgtctgtca
     2521 atcggtgact gtgtgggact tattgaggtg gtgcgaaatt ctcacactat tatgcaaatt
     2581 cagtgcaaag gcggcttgaa aggtgcactg cagttcaaca gccacacact acatcagtgg
     2641 ctcaaagaca agaacaaagg agaaatatat gatgcagcca ttgacctgtt tacacgttca
     2701 tgtgctggat actgtgtagc taccttcatt ttgggaattg gagatcgtca caatagtaac
     2761 atcatggtga aagacgatgg acaactgttt catatagatt ttggacactt tttggatcac
     2821 aagaagaaaa aatttggtta taaacgagaa cgtgtgccat ttgttttgac acaggatttc
     2881 ttaatagtga ttagtaaagg agcccaagaa tgcacaaaga caagagaatt tgagaggttt
     2941 caggagatgt gttacaaggc ttatctagct attcgacagc atgccaatct cttcataaat
     3001 cttttctcaa tgatgcttgg ctctggaatg ccagaactac aatcttttga tgacattgca
     3061 tacattcgaa agaccctagc cttagataaa actgagcaag aggctttgga gtatttcatg
     3121 aaacaaatga atgatgcaca tcatggtggc tggacaacaa aaatggattg gatcttccac
     3181 acaattaaac agcatgcatt gaactga
//
