LOCUS       sequence-157578-        3639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing EGFR, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_EGFR_p.774_775insHV
FEATURES             Location/Qualifiers
     source          1..3639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..72,73..1935,1936..2004,2005..3639)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIG
                     SQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEY
                     HAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILE
                     KGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERM
                     HLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNST
                     VACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSV
                     QNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQ
                     ISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
ORIGIN
        1 atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg
       61 gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag
      121 ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg
      181 gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag
      241 accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct
      301 ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca
      361 gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta
      421 caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg caacgtggag
      481 agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc gatggacttc
      541 cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg
      601 ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca gcagtgctcc
      661 gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc tgcaggctgc
      721 acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga agccacgtgc
      781 aaggacacct gccccccact catgctctac aaccccacca cgtaccagat ggatgtgaac
      841 cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg taattatgtg
      901 gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga gatggaggaa
      961 gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata
     1021 ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa
     1081 aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc
     1141 ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa
     1201 atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt
     1261 gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc
     1321 gtcagcctga acataacatc cttgggatta cgctccctca aggagataag tgatggagat
     1381 gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg
     1441 tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag
     1501 gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc
     1561 agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac
     1621 cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca
     1681 gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc
     1741 cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg
     1801 ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc
     1861 catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg
     1921 cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg
     1981 gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg
     2041 aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga agctcccaac
     2101 caagctctct tgaggatctt gaaggaaact gaattcaaaa agatcaaagt gctgggctcc
     2161 ggtgcgttcg gcacggtgta taagggactc tggatcccag aaggtgagaa agttaaaatt
     2221 cccgtcgcta tcaaggaatt aagagaagca acatctccga aagccaacaa ggaaatcctc
     2281 gatgaagcct acgtgatggc cagcgtggac aacccccacg tccacgtgtg ccgcctgctg
     2341 ggcatctgcc tcacctccac cgtgcaactc atcacgcagc tcatgccctt cggctgcctc
     2401 ctggactatg tccgggaaca caaagacaat attggctccc agtacctgct caactggtgt
     2461 gtgcagatcg caaagggcat gaactacttg gaggaccgtc gcttggtgca ccgcgacctg
     2521 gcagccagga acgtactggt gaaaacaccg cagcatgtca agatcacaga ttttgggctg
     2581 gccaaactgc tgggtgcgga agagaaagaa taccatgcag aaggaggcaa agtgcctatc
     2641 aagtggatgg cattggaatc aattttacac agaatctata cccaccagag tgatgtctgg
     2701 agctacgggg tgaccgtttg ggagttgatg acctttggat ccaagccata tgacggaatc
     2761 cctgccagcg agatctcctc catcctggag aaaggagaac gcctccctca gccacccata
     2821 tgtaccatcg atgtctacat gatcatggtc aagtgctgga tgatagacgc agatagtcgc
     2881 ccaaagttcc gtgagttgat catcgaattc tccaaaatgg cccgagaccc ccagcgctac
     2941 cttgtcattc agggggatga aagaatgcat ttgccaagtc ctacagactc caacttctac
     3001 cgtgccctga tggatgaaga agacatggac gacgtggtgg atgccgacga gtacctcatc
     3061 ccacagcagg gcttcttcag cagcccctcc acgtcacgga ctcccctcct gagctctctg
     3121 agtgcaacca gcaacaattc caccgtggct tgcattgata gaaatgggct gcaaagctgt
     3181 cccatcaagg aagacagctt cttgcagcga tacagctcag accccacagg cgccttgact
     3241 gaggacagca tagacgacac cttcctccca gtgcctgaat acataaacca gtccgttccc
     3301 aaaaggcccg ctggctctgt gcagaatcct gtctatcaca atcagcctct gaaccccgcg
     3361 cccagcagag acccacacta ccaggacccc cacagcactg cagtgggcaa ccccgagtat
     3421 ctcaacactg tccagcccac ctgtgtcaac agcacattcg acagccctgc ccactgggcc
     3481 cagaaaggca gccaccaaat tagcctggac aaccctgact accagcagga cttctttccc
     3541 aaggaagcca agccaaatgg catctttaag ggctccacag ctgaaaatgc agaataccta
     3601 agggtcgcgc cacaaagcag tgaatttatt ggagcatga
//
