LOCUS       sequence-157582-        3399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing TERT, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_TERT_p.L593F
FEATURES             Location/Qualifiers
     source          1..3399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..3399
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHFKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
ORIGIN
        1 atgccgcgcg ctccccgctg ccgagccgtg cgctccctgc tgcgcagcca ctaccgcgag
       61 gtgctgccgc tggccacgtt cgtgcggcgc ctggggcccc agggctggcg gctggtgcag
      121 cgcggggacc cggcggcttt ccgcgcgctg gtggcccagt gcctggtgtg cgtgccctgg
      181 gacgcacggc cgccccccgc cgccccctcc ttccgccagg tgtcctgcct gaaggagctg
      241 gtggcccgag tgctgcagag gctgtgcgag cgcggcgcga agaacgtgct ggccttcggc
      301 ttcgcgctgc tggacggggc ccgcgggggc ccccccgagg ccttcaccac cagcgtgcgc
      361 agctacctgc ccaacacggt gaccgacgca ctgcggggga gcggggcgtg ggggctgctg
      421 ctgcgccgcg tgggcgacga cgtgctggtt cacctgctgg cacgctgcgc gctctttgtg
      481 ctggtggctc ccagctgcgc ctaccaggtg tgcgggccgc cgctgtacca gctcggcgct
      541 gccactcagg cccggccccc gccacacgct agtggacccc gaaggcgtct gggatgcgaa
      601 cgggcctgga accatagcgt cagggaggcc ggggtccccc tgggcctgcc agccccgggt
      661 gcgaggaggc gcgggggcag tgccagccga agtctgccgt tgcccaagag gcccaggcgt
      721 ggcgctgccc ctgagccgga gcggacgccc gttgggcagg ggtcctgggc ccacccgggc
      781 aggacgcgtg gaccgagtga ccgtggtttc tgtgtggtgt cacctgccag acccgccgaa
      841 gaagccacct ctttggaggg tgcgctctct ggcacgcgcc actcccaccc atccgtgggc
      901 cgccagcacc acgcgggccc cccatccaca tcgcggccac cacgtccctg ggacacgcct
      961 tgtcccccgg tgtacgccga gaccaagcac ttcctctact cctcaggcga caaggagcag
     1021 ctgcggccct ccttcctact cagctctctg aggcccagcc tgactggcgc tcggaggctc
     1081 gtggagacca tctttctggg ttccaggccc tggatgccag ggactccccg caggttgccc
     1141 cgcctgcccc agcgctactg gcaaatgcgg cccctgtttc tggagctgct tgggaaccac
     1201 gcgcagtgcc cctacggggt gctcctcaag acgcactgcc cgctgcgagc tgcggtcacc
     1261 ccagcagccg gtgtctgtgc ccgggagaag ccccagggct ctgtggcggc ccccgaggag
     1321 gaggacacag acccccgtcg cctggtgcag ctgctccgcc agcacagcag cccctggcag
     1381 gtgtacggct tcgtgcgggc ctgcctgcgc cggctggtgc ccccaggcct ctggggctcc
     1441 aggcacaacg aacgccgctt cctcaggaac accaagaagt tcatctccct ggggaagcat
     1501 gccaagctct cgctgcagga gctgacgtgg aagatgagcg tgcggggctg cgcttggctg
     1561 cgcaggagcc caggggttgg ctgtgttccg gccgcagagc accgtctgcg tgaggagatc
     1621 ctggccaagt tcctgcactg gctgatgagt gtgtacgtcg tcgagctgct caggtctttc
     1681 ttttatgtca cggagaccac gtttcaaaag aacaggctct ttttctaccg gaagagtgtc
     1741 tggagcaagt tgcaaagcat tggaatcaga cagcacttca agagggtgca gctgcgggag
     1801 ctgtcggaag cagaggtcag gcagcatcgg gaagccaggc ccgccctgct gacgtccaga
     1861 ctccgcttca tccccaagcc tgacgggctg cggccgattg tgaacatgga ctacgtcgtg
     1921 ggagccagaa cgttccgcag agaaaagagg gccgagcgtc tcacctcgag ggtgaaggca
     1981 ctgttcagcg tgctcaacta cgagcgggcg cggcgccccg gcctcctggg cgcctctgtg
     2041 ctgggcctgg acgatatcca cagggcctgg cgcaccttcg tgctgcgtgt gcgggcccag
     2101 gacccgccgc ctgagctgta ctttgtcaag gtggatgtga cgggcgcgta cgacaccatc
     2161 ccccaggaca ggctcacgga ggtcatcgcc agcatcatca aaccccagaa cacgtactgc
     2221 gtgcgtcggt atgccgtggt ccagaaggcc gcccatgggc acgtccgcaa ggccttcaag
     2281 agccacgtct ctaccttgac agacctccag ccgtacatgc gacagttcgt ggctcacctg
     2341 caggagacca gcccgctgag ggatgccgtc gtcatcgagc agagctcctc cctgaatgag
     2401 gccagcagtg gcctcttcga cgtcttccta cgcttcatgt gccaccacgc cgtgcgcatc
     2461 aggggcaagt cctacgtcca gtgccagggg atcccgcagg gctccatcct ctccacgctg
     2521 ctctgcagcc tgtgctacgg cgacatggag aacaagctgt ttgcggggat tcggcgggac
     2581 gggctgctcc tgcgtttggt ggatgatttc ttgttggtga cacctcacct cacccacgcg
     2641 aaaaccttcc tcaggaccct ggtccgaggt gtccctgagt atggctgcgt ggtgaacttg
     2701 cggaagacag tggtgaactt ccctgtagaa gacgaggccc tgggtggcac ggcttttgtt
     2761 cagatgccgg cccacggcct attcccctgg tgcggcctgc tgctggatac ccggaccctg
     2821 gaggtgcaga gcgactactc cagctatgcc cggacctcca tcagagccag tctcaccttc
     2881 aaccgcggct tcaaggctgg gaggaacatg cgtcgcaaac tctttggggt cttgcggctg
     2941 aagtgtcaca gcctgtttct ggatttgcag gtgaacagcc tccagacggt gtgcaccaac
     3001 atctacaaga tcctcctgct gcaggcgtac aggtttcacg catgtgtgct gcagctccca
     3061 tttcatcagc aagtttggaa gaaccccaca tttttcctgc gcgtcatctc tgacacggcc
     3121 tccctctgct actccatcct gaaagccaag aacgcaggga tgtcgctggg ggccaagggc
     3181 gccgccggcc ctctgccctc cgaggccgtg cagtggctgt gccaccaagc attcctgctc
     3241 aagctgactc gacaccgtgt cacctacgtg ccactcctgg ggtcactcag gacagcccag
     3301 acgcagctga gtcggaagct cccggggacg acgctgactg ccctggaggc cgcagccaac
     3361 ccggcactgc cctcagactt caagaccatc ctggactga
//
