LOCUS       sequence-157637-        3618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing EGFR, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_EGFR_p.ELREA_746del
FEATURES             Location/Qualifiers
     source          1..3618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..72,73..1935,1936..2004,2005..3618)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKTSPKANKE
                     ILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNW
                     CVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKV
                     PIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQ
                     PPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTD
                     SNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRN
                     GLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHN
                     QPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPD
                     YQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
ORIGIN
        1 atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg
       61 gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag
      121 ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg
      181 gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag
      241 accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct
      301 ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca
      361 gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta
      421 caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg caacgtggag
      481 agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc gatggacttc
      541 cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg
      601 ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca gcagtgctcc
      661 gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc tgcaggctgc
      721 acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga agccacgtgc
      781 aaggacacct gccccccact catgctctac aaccccacca cgtaccagat ggatgtgaac
      841 cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg taattatgtg
      901 gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga gatggaggaa
      961 gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata
     1021 ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa
     1081 aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc
     1141 ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa
     1201 atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt
     1261 gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc
     1321 gtcagcctga acataacatc cttgggatta cgctccctca aggagataag tgatggagat
     1381 gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg
     1441 tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag
     1501 gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc
     1561 agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac
     1621 cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca
     1681 gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc
     1741 cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg
     1801 ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc
     1861 catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg
     1921 cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg
     1981 gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg
     2041 aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga agctcccaac
     2101 caagctctct tgaggatctt gaaggaaact gaattcaaaa agatcaaagt gctgggctcc
     2161 ggtgcgttcg gcacggtgta taagggactc tggatcccag aaggtgagaa agttaaaatt
     2221 cccgtcgcta tcaaaacatc tccgaaagcc aacaaggaaa tcctcgatga agcctacgtg
     2281 atggccagcg tggacaaccc ccacgtgtgc cgcctgctgg gcatctgcct cacctccacc
     2341 gtgcaactca tcacgcagct catgcccttc ggctgcctcc tggactatgt ccgggaacac
     2401 aaagacaata ttggctccca gtacctgctc aactggtgtg tgcagatcgc aaagggcatg
     2461 aactacttgg aggaccgtcg cttggtgcac cgcgacctgg cagccaggaa cgtactggtg
     2521 aaaacaccgc agcatgtcaa gatcacagat tttgggctgg ccaaactgct gggtgcggaa
     2581 gagaaagaat accatgcaga aggaggcaaa gtgcctatca agtggatggc attggaatca
     2641 attttacaca gaatctatac ccaccagagt gatgtctgga gctacggggt gaccgtttgg
     2701 gagttgatga cctttggatc caagccatat gacggaatcc ctgccagcga gatctcctcc
     2761 atcctggaga aaggagaacg cctccctcag ccacccatat gtaccatcga tgtctacatg
     2821 atcatggtca agtgctggat gatagacgca gatagtcgcc caaagttccg tgagttgatc
     2881 atcgaattct ccaaaatggc ccgagacccc cagcgctacc ttgtcattca gggggatgaa
     2941 agaatgcatt tgccaagtcc tacagactcc aacttctacc gtgccctgat ggatgaagaa
     3001 gacatggacg acgtggtgga tgccgacgag tacctcatcc cacagcaggg cttcttcagc
     3061 agcccctcca cgtcacggac tcccctcctg agctctctga gtgcaaccag caacaattcc
     3121 accgtggctt gcattgatag aaatgggctg caaagctgtc ccatcaagga agacagcttc
     3181 ttgcagcgat acagctcaga ccccacaggc gccttgactg aggacagcat agacgacacc
     3241 ttcctcccag tgcctgaata cataaaccag tccgttccca aaaggcccgc tggctctgtg
     3301 cagaatcctg tctatcacaa tcagcctctg aaccccgcgc ccagcagaga cccacactac
     3361 caggaccccc acagcactgc agtgggcaac cccgagtatc tcaacactgt ccagcccacc
     3421 tgtgtcaaca gcacattcga cagccctgcc cactgggccc agaaaggcag ccaccaaatt
     3481 agcctggaca accctgacta ccagcaggac ttctttccca aggaagccaa gccaaatggc
     3541 atctttaagg gctccacagc tgaaaatgca gaatacctaa gggtcgcgcc acaaagcagt
     3601 gaatttattg gagcatga
//
