LOCUS       sequence-157668-        1599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing GRB7, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_GRB7_WT_V5
FEATURES             Location/Qualifiers
     source          1..1599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(189..200)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggagctgg atctgtctcc acctcatctt agcagctctc cggaagacct ttgcccagcc
       61 cctgggaccc ctcctgggac tccccggccc cctgataccc ctctgcctga ggaggtaaag
      121 aggtcccagc ctctcctcat cccaaccacc ggcaggaaac ttcgagagga ggagaggcgt
      181 gccacctccc tcccctctat ccccaacccc ttccctgagc tctgcagtcc tccctcacag
      241 agcccaattc tcgggggccc ctccagtgca agggggctgc tcccccgcga tgccagccgc
      301 ccccatgtag taaaggtgta cagtgaggat ggggcctgca ggtctgtgga ggtggcagca
      361 ggtgccacag ctcgccacgt gtgtgaaatg ctggtgcagc gagctcacgc cttgagcgac
      421 gagacctggg ggctggtgga gtgccacccc cacctagcac tggagcgggg tttggaggac
      481 cacgagtccg tggtggaagt gcaggctgcc tggcccgtgg gcggagatag ccgcttcgtc
      541 ttccggaaaa acttcgccaa gtacgaactg ttcaagagct ccccacactc cctgttccca
      601 gaaaaaatgg tctccagctg tctcgatgca cacactggta tatcccatga agacctcatc
      661 cagaacttcc tgaatgctgg cagctttcct gagatccagg gctttctgca gctgcggggt
      721 tcaggacgga agctttggaa acgctttttc tgcttcttgc gccgatctgg cctctattac
      781 tccaccaagg gcacctctaa ggatccgagg cacctgcagt acgtggcaga tgtgaacgag
      841 tccaacgtgt acgtggtgac gcagggccgc aagctctacg ggatgcccac tgacttcggt
      901 ttctgtgtca agcccaacaa gcttcgaaat ggccacaagg ggcttcggat cttctgcagt
      961 gaagatgagc agagccgcac ctgctggctg gctgccttcc gcctcttcaa gtacggggtg
     1021 cagctgtaca agaattacca gcaggcacag tctcgccatc tgcatccatc ttgtttgggc
     1081 tccccaccct tgagaagtgc ctcagataat accctggtgg ccatggactt ctctggccat
     1141 gctgggcgtg tcattgagaa cccccgggag gctctgagtg tggccctgga ggaggcccag
     1201 gcctggagga agaagacaaa ccaccgcctc agcctgccca tgccagcctc cggcacgagc
     1261 ctcagtgcag ccatccaccg cacccaactc tggttccacg ggcgcatttc ccgtgaggag
     1321 agccagcggc ttattggaca gcagggcttg gtagacggcc tgttcctggt ccgggagagt
     1381 cagcggaacc cccagggctt tgtcctctct ttgtgccacc tgcagaaagt gaagcattat
     1441 ctcatcctgc cgagcgagga ggagggccgc ctgtacttca gcatggatga tggccagacc
     1501 cgcttcactg acctgctgca gctcgtggag ttccaccagc tgaaccgcgg catcctgccg
     1561 tgcttgctgc gccattgctg cacgcgggtg gccctctga
//
