LOCUS       sequence-157717-        2544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PYGL, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_PYGL_WT_V5
FEATURES             Location/Qualifiers
     source          1..2544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcgaagc ccctgacgga ccaggagaag cggcggcaga tcagcatccg cggcatcgtg
       61 ggcgtggaga acgtggcaga gctgaagaag agtttcaacc ggcacctgca cttcacgctg
      121 gtcaaggacc gcaacgtggc caccacccgc gactactact tcgcgctggc gcacacggtg
      181 cgcgaccacc tggtggggcg ctggatccgc acgcagcagc actactacga caagtgcccc
      241 aagagggtat attacctctc tctggaattt tacatgggcc gaacattaca gaacaccatg
      301 atcaacctcg gtctgcaaaa tgcctgtgat gaggccattt accagcttgg attggatata
      361 gaagagttag aagaaattga agaagatgct ggacttggca atggtggtct tgggagactt
      421 gctgcctgct tcttggattc catggcaacc ctgggacttg cagcctatgg atacggcatt
      481 cggtatgaat atgggatttt caatcagaag atccgagatg gatggcaggt agaagaagca
      541 gatgattggc tcagatatgg aaacccttgg gagaagtccc gcccagaatt catgctgcct
      601 gtgcacttct atggaaaagt agaacacacc aacaccggga ccaagtggat tgacactcaa
      661 gtggtcctgg ctctgccata tgacaccccc gtgcccggct acatgaataa cactgtcaac
      721 accatgcgcc tctggtctgc tcgggcacca aatgacttta acctcagaga ctttaatgtt
      781 ggagactaca ttcaggctgt gctggaccga aacctggccg agaacatctc ccgggtcctc
      841 tatcccaatg acaatttttt tgaagggaag gagctaagat tgaagcagga atactttgtg
      901 gtggctgcaa ccttgcaaga tatcatccgc cgtttcaaag cctccaagtt tggctccacc
      961 cgtggtgcag gaactgtgtt tgatgccttc ccggatcagg tggccatcca gctgaatgac
     1021 actcaccctg cactcgcgat ccctgagctg atgaggattt ttgtggatat tgaaaaactg
     1081 ccctggtcca aggcatggga gctcacccag aagaccttcg cctacaccaa ccacacagtg
     1141 ctcccggaag ccctggagcg ctggcccgtg gacctggtgg agaagctgct ccctcgacat
     1201 ttggaaatca tttatgagat aaatcagaag catttagata gaattgtggc cttgtttcct
     1261 aaagatgtgg accgtctgag aaggatgtct ctgatagaag aggaaggaag caaaaggatc
     1321 aacatggccc atctctgcat tgtcggttcc catgctgtga atggcgtggc taaaatccac
     1381 tcagacatcg tgaagactaa agtattcaag gacttcagtg agctagaacc tgacaagttt
     1441 cagaataaaa ccaatgggat cactccaagg cgctggctcc tactctgcaa cccaggactt
     1501 gcagagctca tagcagagaa aattggagaa gactatgtga aagacctgag ccagctgacg
     1561 aagctccaca gcttcctggg tgatgatgtc ttcctccggg aactcgccaa ggtgaagcag
     1621 gagaataagc tgaagttttc tcagttcctg gagacggagt acaaagtgaa gatcaaccca
     1681 tcctccatgt ttgatgtcca ggtgaagagg atacatgagt acaagcgaca gctcttgaac
     1741 tgtctgcatg tgatcacgat gtacaaccgc attaagaaag accctaagaa gttattcgtg
     1801 ccaaggacag ttatcattgg tggtaaagct gccccaggat atcacatggc caaaatgatc
     1861 ataaagctga tcacttcagt ggcagatgtg gtgaacaatg accctatggt tggaagcaag
     1921 ttgaaagtca tcttcttgga gaactacaga gtatctcttg ctgaaaaagt cattccagcc
     1981 acagatctgt cagagcagat ttccactgca ggcaccgaag cctcggggac aggcaatatg
     2041 aagttcatgc taaatggggc cctaactatc gggaccatgg atggggccaa tgtggaaatg
     2101 gcagaagaag ctggggaaga gaacctgttc atctttggca tgaggataga tgatgtggct
     2161 gctttggaca agaaagggta cgaggcaaaa gaatactatg aggcacttcc agagctgaag
     2221 ctggtcattg atcaaattga caatggcttt ttttctccca agcagcctga cctcttcaaa
     2281 gatatcatca acatgctatt ttatcatgac aggtttaaag tctttgcaga ctacgaagcc
     2341 tatgtcaagt gtcaagataa agtgagtcag ctgtacatga atccaaaggc ctggaacaca
     2401 atggtactca aaaacatagc tgcctcgggg aaattctcca gtgaccgaac aattaaagaa
     2461 tatgcccaaa acatctggaa cgtggaacct tcagatctaa agatttctct atccaatgaa
     2521 tctaacaaag tcaatggaaa ttga
//
