LOCUS       sequence-157745-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of constitutively active ALK5 T204D .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Moren A, Imamura T, Miyazono K, Heldin CH, Moustakas A
  TITLE     Degradation of the tumor suppressor Smad4 by WW and HECT domain 
            ubiquitin ligases.
  JOURNAL   J Biol Chem. 2005 Jun 10;280(23):22115-23. Epub 2005 Apr 6.
  PUBMED    15817471
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-06-10"; volume: "280(23)"; pages: "22115-23. 
            Epub 2005 Apr 6"
COMMENT     Sequence Label: pcDNA3-ALK5 T204D
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcgactctc tggctactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg
      121 tgctggaatt cgggccatgg aggcggcggt cgctgctccg cgtccccggc tgctcctcct
      181 cgtgctggcg gcggcggcgg cggcggcggc ggcgctgctc ccgggggcga cggcgttaca
      241 gtgtttctgc cacctctgta caaaagacaa ttttacttgt gtgacagatg ggctctgctt
      301 tgtctctgtc acagagacca cagacaaagt tatacacaac agcatgtgta tagctgaaat
      361 tgacttaatt ccacgagata ggccgtttgt atgtgcaccc tcttcaaaaa ctgggtctgt
      421 gactacaaca tattgctgca atcaggacca ttgcaataaa atagaacttc caactactgt
      481 aaagtcatca cctggccttg gtcctgtgga actggcagct gtcattgctg gaccagtgtg
      541 cttcgtctgc atctcactca tgttgatggt ctatatctgc cacaaccgca ctgtcattca
      601 ccatcgagtg ccaaatgaag aggacccttc attagatcgc ccttttattt cagagggtac
      661 tacgttgaaa gacttaattt atgatatgac aacgtcaggt tctggctcag gtttaccatt
      721 gcttgttcag agaacaattg cgagagatat tgtgttacaa gaaagcattg gcaaaggtcg
      781 atttggagaa gtttggagag gaa
//
