LOCUS       sequence-157746-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of constitutively active ALK5 T204D .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Moren A, Imamura T, Miyazono K, Heldin CH, Moustakas A
  TITLE     Degradation of the tumor suppressor Smad4 by WW and HECT domain 
            ubiquitin ligases.
  JOURNAL   J Biol Chem. 2005 Jun 10;280(23):22115-23. Epub 2005 Apr 6.
  PUBMED    15817471
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-06-10"; volume: "280(23)"; pages: "22115-23. 
            Epub 2005 Apr 6"
COMMENT     Sequence Label: pcDNA3-ALK5 T204D
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..59)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(39..65)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cctatagaat agggccctct agagtcgacc tagaggctag cataatcagg aacatcatac
       61 ggataaccct cgagcatttt gatgccttcc tgttgactga gttgcgataa tgttttctta
      121 atccgcaatg ctgtaagcct agctgctcca ttggcatacc aacattctct cataatttta
      181 gccattactc tcaaggcttc acagctctgc catctgtttg ggatatttgg ccttaacttc
      241 tgttcacaaa caacttttct catttcttca actgatgggt cagaaggtac aagatcataa
      301 taaggcagtt ggtaatcttc atgaatacca ccaatggaac atcgtcgagc aatttcccag
      361 aatactaagc ccattgcata gatgtcagca cgtttgaagg attcaaaatg tttcatattt
      421 atggaatcat cgagaacttc aggggccatg tacctttttg ttcccactct gtggtttgga
      481 gcaatatcaa tggtatctgt ggctgaatca tgtcttactg ccagtcctaa gtctgcaata
      541 cagcaagttc cattcttctt taccaagata ttctttgatt tcaaatctct atgagcaatg
      601 gctggctttc cttgggtacc aacaatctcc atgtgaagat gggcaagacc gctcgccgtg
      661 gacagagcaa gttttatcat tccttccaca gtaactgtgt atctgtttaa gtaatcaaaa
      721 agggatccat gctcatgata atctgacacc aaccagagct gagtccaagt accattgtct
      781 ttattgtctg ctgctataaa tcccaggatg tt
//
