LOCUS       sequence-157747-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of ALK5 K232R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Moren A, Imamura T, Miyazono K, Heldin CH, Moustakas A
  TITLE     Degradation of the tumor suppressor Smad4 by WW and HECT domain 
            ubiquitin ligases.
  JOURNAL   J Biol Chem. 2005 Jun 10;280(23):22115-23. Epub 2005 Apr 6.
  PUBMED    15817471
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-06-10"; volume: "280(23)"; pages: "22115-23. 
            Epub 2005 Apr 6"
COMMENT     Sequence Label: pcDNA3-ALK5 K232R
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ccactagtaa cggccgccag
      121 tgtgctggaa ttcgggccat ggaggcggcg gtcgctgctc cgcgtccccg gctgctcctc
      181 ctcgtgctgg cggcggcggc ggcggcggcg gcggcgctgc tcccgggggc gacggcgtta
      241 cagtgtttct gccacctctg tacaaaagac aattttactt gtgtgacaga tgggctctgc
      301 tttgtctctg tcacagagac cacagacaaa gttatacaca acagcatgtg tatagctgaa
      361 attgacttaa ttccacgaga taggccgttt gtatgtgcac cctcttcaaa aactgggtct
      421 gtgactacaa catattgctg caatcaggac cattgcaata aaatagaact tccaactact
      481 gtaaagtcat cacctggcct tggtcctgtg gaactggcag ctgtcattgc tggaccagtg
      541 tgcttcgtct gcatctcact catgttgatg gtctatatct gccacaaccg cactgtcatt
      601 caccatcgag tgccaaatga agaggaccct tcattagatc gcccttttat ttcagagggt
      661 actacgttga aagacttaat ttatgatatg acaacgtcag gttctggctc aggtttacca
      721 ttgcttgttc agagaacaat tgcgagaact attgtgttac aagaaagcat tggcaaaggt
      781 cgatttggag aagtttggag aggaaagtgg cggggagaag aagttgctgt taggatattc
      841 tcctctagag aagaacgttc gtg
//
