LOCUS       sequence-157759-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Vpr binding mutant EPN-01*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-01*-(Delta-LF)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(223..252)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aggggcttca tgatgtcccc ataatttttg gcagagggaa aaagatctgc tagcaagctt
       61 ctcgagtcac tgagaagacg ggtcgttacc ggccgcagaa cgcagagagg tcagcgggta
      121 cagttctttg tcgatcggct cctgtttttg cggtggcgtg gtggtttcaa caccagaacg
      181 gaaagattct tccggcggtg cggtcggttc cggacgagac tgcagatctt cttcgctaat
      241 cagtttctgt tcggtgcagc cacgaatctt ctcaacgaac gccttggcct tttccgccac
      301 ttccaccggg gtgcctttca ctaacgcgct gcccacgccc acggccagca cgcccgcttt
      361 gaaccattcg cacacattgt ccagattaac accgccggtt ggcacaaatt tcacattcgg
      421 aaacggacct ttcatcgctt taacaaactg cggacccacc acctcacccg gaaacagttt
      481 taagatggta tggcccagtt tcatggcttt caccagttcg gtcggggtca taacgcctgg
      541 catatagaac acacccttct ccttgcaaaa ttggctaatt tcctcgtcca ggtgcggact
      601 cacaatgaat tcggcgccac tttccaccgc tttacggcac tgctccacgc tggtcacggt
      661 gcccgcacca ataatcgcgc ccatttcctt cagaaagctc agttccttaa tcacggtatc
      721 cgcatccggc acggtaaagg taatttcaat cagatgaacg ccgcccagga aaaccgccag
      781 cgctttcttt ttcgcttctt ccacactatt ggcacgcagc acggcaacga tcttgtgttt
      841 cttgaacagc tcttccattt tgctac
//
