LOCUS       sequence-157771-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Blakeley BD, Chapman AM, McNaughton BR
  TITLE     Split-superpositive GFP reassembly is a fast, efficient, and robust 
            method for detecting protein-protein interactions in vivo.
  JOURNAL   Mol Biosyst. 2012 Aug;8(8):2036-40. Epub 2012 Jun 12.
  PUBMED    22692102
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biosyst."; date: "2012-08"; volume: "8(8)"; pages: "2036-40. Epub 
            2012 Jun 12"
COMMENT     Sequence Label: pET11a-Z-NspGFP
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             599..610
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(688..706)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      702..749
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgggtca tcaccaccac catcacggtg gcgctagcaa
       61 aggtgaacgt ctgtttcgtg gtaaagtacc gatcttagtg gaattaaagg gcgacgtgaa
      121 cggtcataaa tttagcgtgc gcggcgaagg caaaggtgac gctacccgtg gtaaattgac
      181 cctgaagttt atttgcacaa caggcaaatt acccgttccg tggcccacct tagtgaccac
      241 cctgacctat ggcgttcagt gcttcagtcg ttaccctaaa catatgaaac gtcacgattt
      301 tttcaaatca gccatgccta aaggatatgt tcaagagcgt acaatcagct tcaagaagga
      361 tggcaaatat aaaacgcgtg cggaagtgaa atttgaaggc cgcacactag taaatcgtat
      421 caaactgaaa ggtcgtgact tcaaagaaaa aggcaacatt ttaggccata aactgcgtta
      481 taactttaat tctcataagg tgtatattac ggccgataaa cgcggtggct ctggctctgg
      541 ctcgagcgcc ctcaaaaaag aattgcaggc aaacaaaaaa gaacttgcgc agctgaagtg
      601 ggagttacaa gctctgaaaa aggaactggc gcagtaagga tccggctgct aacaaagccc
      661 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      721 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatcccg
      781 caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt
      841 gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca
      901 atttaactgt
//
